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  • 2017 (1)

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  • Ambrose, Christine (1)
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  • Rademakers, Rosa
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  • Neurology: Neuromuscular

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Article

Poly(GP) proteins are a useful pharmacodynamic marker for C9ORF72-associated amyotrophic lateral sclerosis

by Tania F. Gendron; Jeannie Chew; Jeannette N. Stankowski; Lindsey R. Hayes; Yong-Jie Zhang; Mercedes Prudencio; Yari Carlomagno; Lillian M. Daughrity; Karen Jansen-West; Emilie A. Perkerson; Aliesha O'Raw; Casey Cook; Luc Pregent; Veronique Belzil; Marka van Blitterswijk; Lilia J. Tabassian; Chris W. Lee; Mei Yue; Jimei Tong; Yuping Song; Monica Castanedes-Casey; Linda Rousseau; Virginia Phillips; Dennis W. Dickson; Rosa Rademakers; John D. Fryer; Beth K. Rush; Otto Pedraza; Ana M. Caputo; Pamela Desaro; Carla Palmucci; Amelia Robertson; Michael G. Heckman; Nancy N. Diehl; Edythe Wiggs; Michael Tierney; Laura Braun; Jennifer Farren; David Lacomis; Shafeeq Ladha; Christina Nicole Fournier; Leo F. McCluskey; Lauren B. Elman; Jon B. Toledo; Jennifer D. McBride; Cinzia Tiloca; Claudia Morelli; Barbara Poletti; Federica Solca; Alessandro Prelle; Joanne Wuu; Jennifer Jockel-Balsarotti; Frank Rigo; Christine Ambrose; Abhishek Datta; Weixing Yang; Denitza Raitcheva; Giovanna Antognetti; Alexander McCampbell; John C. Van Swieten; Bruce L. Miller; Adam L. Boxer; Robert H. Brown; Robert Bowser; Timothy Miller; John Q. Trojanowski; Murray Grossman; James D. Berry; William Hu; Antonia Ratti; Bryan J. Traynor; Matthew D. Disney; Michael Benatar; Vincenzo Silani; Jonathan D Glass; Mary Kay Floeter; Jeffrey D. Rothstein; Kevin B. Boylan; Leonard Petrucelli

2017

Subjects
  • Biology, Neuroscience
  • Health Sciences, Medicine and Surgery
  • Health Sciences, Pathology
  • File Download
  • View Abstract

Abstract:Close

There is no effective treatment for amyotrophic lateral sclerosis (ALS), a devastating motor neuron disease. However, discovery of a G 4 C 2 repeat expansion in the C9ORF72 gene as the most common genetic cause of ALS has opened up new avenues for therapeutic intervention for this form of ALS. G 4 C 2 repeat expansion RNAs and proteins of repeating dipeptides synthesized from these transcripts are believed to play a key role in C9ORF72-associated ALS (c9ALS). Therapeutics that target G 4 C 2 RNA, such as antisense oligonucleotides (ASOs) and small molecules, are thus being actively investigated. A limitation in moving such treatments from bench to bedside is a lack of pharmacodynamic markers for use in clinical trials. We explored whether poly(GP) proteins translated from G 4 C 2 RNA could serve such a purpose. Poly(GP) proteins were detected in cerebrospinal fluid (CSF) and in peripheral blood mononuclear cells from c9ALS patients and, notably, from asymptomatic C9ORF72 mutation carriers. Moreover, CSF poly(GP) proteins remained relatively constant over time, boding well for their use in gauging biochemical responses to potential treatments. Treating c9ALS patient cells or a mouse model of c9ALS with ASOs that target G 4 C 2 RNA resulted in decreased intracellular and extracellular poly(GP) proteins. This decrease paralleled reductions in G 4 C 2 RNA and downstream G 4 C 2 RNA-mediated events. These findings indicate that tracking poly(GP) proteins in CSF could provide a means to assess target engagement of G 4 C 2 RNA-based therapies in symptomatic C9ORF72 repeat expansion carriers and presymptomatic individuals who are expected to benefit from early therapeutic intervention. 2017
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