Mass mortality event in South American sea lions (Otaria flavescens) correlated to highly pathogenic avian influenza (HPAI) H5N1 outbreak in Chile

• Authors: Mauricio Ulloa, University of Las Palmas de Gran Canaria; Antonio Fernández, University of Las Palmas de Gran Canaria; Naomi Ariyama, Universidad de Chile; Ana Colom-Rivero, University of Las Palmas de Gran Canaria; Carlos Rivera, Servicio Agrícola y Ganadero; Paula Nuñez, Servicio Agrícola y Ganadero; Paola Sanhueza, Servicio Agrícola y Ganadero; Magdalena Johow, Servicio Agrícola y Ganadero; Hugo Araya, Servicio Agrícola y Ganadero; Juan Carlos Torres, Servicio Agrícola y Ganadero; Paola Gomez, Servicio Nacional de Pesca y Acuicultura; Gabriela Muñoz, Universidad de Chile; Belén Agüero, Universidad de Chile; Raúl Alegría, Universidad Santo Tomas; Rafael A. Medina, Emory University; Victor Neira, Universidad de Chile; Eva Sierra, University of Las Palmas de gran Canaria
• Journal Title: Veterinary Quarterly
• Volume: Volume 43, Number 1
• Publisher: Taylor & Francis Group | 2023-10-19, Pages 1-10
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1080/01652176.2023.2265173
• Subjects: Biology, Veterinary Science; Health Sciences, Epidemiology; Health Sciences, Public Health
• Keywords: Mortality; South American sea lions; strandings; highly pathogenic avian influenza H5N1; outbreak; Chile

Abstract

In Chile, since January 2023, a sudden and pronounced increase in strandings and mortality has been observed among South American (SA) sea lions (Otaria flavescens), prompting significant concern. Simultaneously, an outbreak of highly pathogenic avian influenza H5N1 (HPAIV H5N1) in avian species has emerged since December 2022. To investigate the cause of this unexpected mortality, we conducted a comprehensive epidemiological and pathologic study. One hundred sixty-nine SA sea lions were sampled to ascertain their HPAIV H5N1 status, and long-term stranding trends from 2009 to 2023 were analyzed. In addition, two animals were necropsied. Remarkably, a significant surge in SA sea lion strandings was observed initiating in January 2023 and peaking in June 2023, with a count of 4,545 stranded and deceased animals. Notably, this surge in mortality correlates geographically with HPAIV outbreaks affecting wild birds. Among 168 sampled SA sea lions, 34 (20%) tested positive for Influenza A virus, and 21 confirmed for HPAIV H5N1 2.3.4.4b clade in tracheal/rectal swab pools. Clinical and pathological evaluations of the two necropsied stranded sea lions revealed prevalent neurological and respiratory signs, including disorientation, tremors, ataxia, and paralysis, as well as acute dyspnea, tachypnea, profuse nasal secretion, and abdominal breathing. The lesions identified in necropsied animals aligned with observed clinical signs. Detection of the virus via immunohistochemistry (IHC) and real-time PCR in the brain and lungs affirmed the findings. The findings provide evidence between the mass mortality occurrences in SA sea lions and HPAIV, strongly indicating a causal relationship. Further studies are needed to better understand the pathogenesis and transmission.