Differential expression of chemokine receptors on monocytes in TB and HIV S

• Authors: Wegene Tamene, Ethiopian Public Health Institute, Addis Ababa; Vincent C Marconi, Emory University; Meseret Abebe, Armauer Hansen Research Institute, Addis Ababa; Liya Wassie, Armauer Hansen Research Institute, Addis Ababa; Yohannes Belay, Ethiopian Public Health Institute, Addis Ababa; Amha Kebede, Ethiopian Public Health Institute, Addis Ababa; Ulrich Sack, Leipzig University; Rawleigh Howe, rmauer Hansen Research Institute, Addis Ababa
• Journal Title: HELIYON
• Volume: Volume 9, Number 6
• Publisher: CELL PRESS | 2023-06-01, Pages e17202-e17202
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1016/j.heliyon.2023.e17202
• Subjects: Health Sciences, Public Health; Health Sciences, Immunology; Health Sciences, Medicine and Surgery
• Keywords: Science & Technology; Multidisciplinary Sciences; Science & Technology - Other Topics; Monocyte subset; Chemokine receptor; TB; HIV; PREFERENTIALLY HARBORS HIV-1; MYCOBACTERIUM-TUBERCULOSIS; IMMUNE ACTIVATION; PULMONARY; INFECTION; CELLS; CCR2; HETEROGENEITY; TRAFFICKING; DEFICIENCY

Abstract

In the present study, we defined multiple chemokine receptors expressed by classical, intermediate and non-classical monocyte subsets in TB, HIV and TB/HIV co-infection and associate it with the perturbation of monocyte subsets due to the diseases. Peripheral blood mononuclear cells from TB+ (n = 34), HIV+ (n = 35), TB + HIV+ (n = 12), as well as TB-HIV- healthy controls (n = 39), were tested for monocyte phenotyping by flow cytometry. Frequencies of intermediate and non-classical monocytes were significantly higher in TB and/or HIV disease relative to healthy controls. CCR2 and CX3CR1 were significantly higher on monocytes in TB disease, whereas CCR4 and CCR5 were present at higher levels in HIV disease. TB/HIV co-infected patients exhibited CCR2, CCR5 and CX3CR1 levels intermediate to TB and HIV subjects, while CCR4 was at a higher level than HIV. Despite the increase in the expression of chemokine receptors due to disease conditions, chemokine receptors maintained their original expression pattern on monocyte subsets. Our data provided new insight into the disease-specific but not monocyte subsets-specific modulation of chemokine receptors in TB and HIV.