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Andreas Wieland, andreas.wieland@emory.edu
Rafi Ahmed, rahmed@emory..edu
C.S.E. performed most of the experiments and analysed the data. H.T.K. analysed the scRNA-seq data. M.R.P. collected and provided human specimens, and analysed patient data. M.A.C. analysed scRNA-seq data. M.A.C., N.P. and A.W. performed in vitro proliferation experiments. R.C.O. performed and analysed multiplex immunohistochemistry experiments. T.H.N. performed flow cytometry experiments. C.C.G. and X.W., supervised by D.M.S., handled human specimens. M.C. performed HLA-typing analyses. J.S. and A.S. performed peptide–MHC affinity measurements. D.M.S., N.F.S. and Z.G.C. initiated the clinical specimen protocol. A.W. processed human specimens. A.W. and R.A. conceived, designed and supervised the project, and contributed equally to this work. C.S.E., H.T.K., A.W. and R.A. wrote the manuscript, with all authors contributing to the revision of the manuscript.
This work was supported by funding from the Ambrose Monell Foundation (R.A.); the T. J. Martell Foundation (R.A.); NIH grants 5U19AI057266 and P01AI056299 (R.A.); the Oliver S. and Jennie R. Donaldson Charitable Trust (R.A.); a Winship Invest$ Pilot grant (R.A., Z.G.C. and N.F.S.); Swiss National Science Foundation grant P300PB_174483 (C.S.E.); a Eugenio Litta Foundation grant (C.S.E.); NCI grant 1-R00-CA197891 (H.T.K.); the James M. Cox Foundation and James C. Kennedy (H.T.K.); the Prostate Cancer Foundation (H.T.K. and N.P.); and a Triological Society Research Career Development Award (M.R.P.). We thank H. Wu for technical assistance and M. Clayton for administrative support. We acknowledge the Emory Flow Cytometry Core supported by the National Center for Georgia Clinical and Translational Science Alliance of the NIH under award number UL1TR002378; the Integrated Cellular Imaging Microscopy Core of the Winship Cancer Institute of Emory University and the NIH-NCI under award number, 2P30CA138292-04; and the Yerkes NHP Genomics Core, which is supported in part by NIH P51 OD011132. This research was supported in part by the Intramural Research Program of the NIH, the Frederick National Laboratory for Cancer Research and the Center for Cancer Research. The project has been funded in part with federal funds from the Frederick National Laboratory for Cancer Research, under contract no. HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products or organizations imply endorsement by the US Government.
R.A. holds patents related to the PD-1 inhibitory pathway. C.S.E., A.W. and R.A. are inventors on a patent application filed by Emory University relating to the use of HPV-specific TCR sequences. All other authors declare no competing interests.