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Sampath Prahalad, Email: sprahal@emory.edu
KI performed most of the single cell data analysis, with assistance from MD and VP. NT conceived the study, recruited patients, supervised the sample acquisition and phenotyping. LP recruited patients and organized participant data. NT and TG were responsible for specimen processing, cell preparation and TNF stimulation, with advice and support of AK, under the supervision of EG who also helped in study design. AK and DA were responsible for generation of single-cell gene expression libraries. SC helped with study design and interpretation of results. SP conceived the study, provided clinical oversight, and edited the manuscript. GG wrote the first draft of the manuscript and supervised the analyses. All authors read and approved the final manuscript.
In addition to the generous participation of all donors, we gratefully acknowledge discussions with James R. Rose while he was rotating in the Ghosn lab, Subra Kugathasan for critical input. We acknowledge Drs. Amit Thakral, Elaine Flanagan and Angela Taneja for referring subjects to the study. Savannah Washburn kindly assisted with the data deposition to GEO. We acknowledge the Molecular Evolution Core the Parker H. Petit Institute for Bioengineering and Bioscience at the Georgia Institute of Technology for the single-cell library sequencing; and Emory Integrated Genomics Core (EIGC), which is subsidized by the Emory University School of Medicine and is one of the Emory Integrated Core Facilities for performing QC on single-cell libraries.
Dr. Prahalad has served on a macrophage activation syndrome adjudication committee for Novartis, but this had no bearing on the work described in this manuscript. None of the other authors have any competing interests in the manuscript.
Kathleen J. Imbach and Nicole J. Treadway contributed equally to this work. Sampath Prahalad and Greg Gibson are shared senior authors.
This work was supported by Marcus Foundation funds awarded to SP, and a Children’s Healthcare of Atlanta, Pediatric Research Alliance seed grant to EG, whose group is partially supported by start-up from the Lowance Center for Human Immunology at Emory University. KJI was a Beckman Foundation undergraduate research scholar, and NJT was partially supported by a Rheumatology Research Foundation medical and graduate student preceptorship award.
© The Author(s) 2023