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Email: arnold@cabm.rutgers.edu
Each author’s contributions to the paper are as follows: Conceptualization: E.A., P.R.G., F.X.R., R.Y., V.V.C., and S.K.D. Methodology: R.Y., V.V.C., S.K.D., J.J.E.K.H., J.T., J.B.H., F.X.R., and R.L.S. Investigation: R.Y., V.V.C., S.K.D., R.L.S., S.G.S., E.A., P.R.G., and F.X.R. Visualization: R.Y., V.V.C., S.K.D., and F.X.R. Supervision: E.A., P.R.G., and F.X.R. Writing—original draft: R.Y., V.V.C., F.X.R., E.A., and P.R.G. Writing—review and editing: All authors.
We thank M. Narwal and K. Murakami (Pennsylvania State University) for providing protein samples of the C145A Mpro mutant and the nsp7-10 polyprotein, numerous helpful discussions, and also critical reading of the manuscript. We thank B. Pascal and R. Vera Alvarez (Omics Informatics LLC) for help in running the nsp7-11 and nsp7-8 models through the HDXer software. We thank H. Zheng and P. Lobel (Biological Mass Spectrometry Facility of Robert Wood Johnson Medical School and Rutgers University) for performing the in-gel LC-MS/MS for the limited proteolysis assay of nsp7-11 polyprotein by Mpro. Molecular graphics and analyses were performed with (i) UCSF Chimera, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco; and (ii) PyMOL [DeLano WL (2002), The PyMOL Molecular Graphics System, San Carlos, CA; DeLano Scientific].
The authors declare that they have no competing interests.
We are grateful for support from National Institutes of Health (NIH) grants U54 AI150472 (to E.A. and P.R.G.), AI 027690 (to E.A.), F31 DK126394 (to V.V.C.), R01 AI167356 (to S.G.S.), and T32 AI157855 (to R.L.S.) and a research award from the Rutgers Center for COVID-19 Response and Pandemic Preparedness (to E.A.).
We are grateful to V. Nanda and P. Falkowski for providing financial support for J.T.’s efforts. This research used resources of the Advanced Photon Source, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under contract no. DE-AC02-06CH11357. This project was supported by grant P30 GM138395 from the National Institute of General Medical Sciences of the NIH.
Use of the Pilatus 3 1M detector was provided by grant 1S10OD018090-01 from NIGMS. The content is solely the responsibility of the authors and does not necessarily reflect the official views of the National Institute of General Medical Sciences or the NIH.
© 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).