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Edward H. Egelman, gelman@virginia.edu
Vincent P. Conticello, vcontic@emory.edu
E.H.E. and V.P.C. designed research; M.A.B.K., S.W., L.C.B., A.T., X.Z., E.H.E., and V.P.C. performed research; M.A.B.K., L.C.B., E.H.E., and V.P.C. analyzed data; and M.A.B.K., E.H.E., and V.P.C. wrote the paper.
Cryo-EM imaging of the PSMα3 and PSMβ2 filaments was performed at the Cryo-EM facility at the National Cancer Institute’s Frederick Laboratory for Cancer Research under Contract No. HSSN261200800001E. This study was supported by the Robert P. Apkarian Integrated Electron Microscopy Core at Emory University, which is subsidized by the School of Medicine and Emory College of Arts and Sciences. Additional support was provided by the Georgia Clinical & Translational Science Alliance of the NIH under award number UL1TR000454. Negative-stain TEM images were gathered on a Hitachi HT7700 120kV TEM at Emory University, which was supported by the Georgia Clinical & Translational Science Alliance under award No. UL1TR002378. This research was supported by grants from the NSF (DMR-1534317) to V.P.C. and the NIH (GM122510) to E.H.E. The CD spectropolarimeter was acquired through funding from an NSF grant (DBI-1726544). M.A.B.K. acknowledges financial support from NIH T32 GM080186 and the University of Virginia Robert R. Wagner Fellowship. L.C.B. acknowledges financial support from NIH T32 AI007046. This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science User Facility, operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. We thank Dr. Joseph Wall of Brookhaven National Laboratory for acquisition of the STEM imaging data.
The authors declare no competing interest.
© 2022 the Author(s). Published by PNAS