61 Views | 28 Downloads
Edward H. Egelman, Email: egelman@virginia.edu
Vincent P. Conticello, vcontic@emory.edu
Conceptualization, F.W., O.G., E.H.E., and V.P.C.; methodology, F.W., E.H.E., and V.P.C.; investigation, F.W., O.G., S.W., T.O., and X.Z.; supervision, E.H.E. and V.P.C.; validation, F.W. and E.H.E.; visualization, F.W., E.H.E., and V.P.C.; writing – original draft, F.W., E.H.E., and V.P.C.; writing – review and editing, F.W., E.H.E., and V.P.C.; funding acquisition, F.W., E.H.E., and V.P.C.
Cryo-EM imaging was conducted at the Molecular Electron Microscopy Core facility at the University of Virginia, which is supported by the School of Medicine and built with National Institutes of Health (NIH) grant G20-RR31199. In addition, the Titan Krios (SIG S10-RR025067) and K3/GIF (U24-GM116790) were purchased, in part or in full, with the designated NIH grants. This study was supported by the Robert P. Apkarian Integrated Electron Microscopy Core (IEMC) at Emory University, which is subsidized by the School of Medicine and Emory College of Arts and Sciences. Negative stain TEM images were acquired on a Hitachi HT7700 120kV TEM at Emory University, which was supported by the Georgia Clinical and Translational Science Alliance under award number UL1TR002378. The content is solely the responsibility of the authors and does not necessarily reflect the official views of the NIH. This research was supported by grants from the National Science Foundation (NSF) (DMR-1534317) to V.P.C., the NIH (GM138756) to F.W., and the NIH (GM122510) to E.H.E. The circular dichroism spectropolarimeter was acquired through funding from an NSF grant (DBI-1726544). This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science User Facility, operated for the DOE Office of Science by Argonne National Laboratory under contract no. DE-AC02-06CH11357. The authors thank Dr. Gevorg Grigoryan for useful discussions
The authors declare no competing interests.