About this item:

18 Views | 6 Downloads

Author Notes:

Email: bilal.haider@bme.gatech.edu

B.W. built the data base, wrote analysis code, and analyzed all experiments; J.D.R., K.P., T.M., A. S., performed silicon probe experiments; S.C., E. K. M. performed patch-clamp experiments, B.W., S.C., T.M., K.P., L. M-B., B.H. performed data analysis; B.W., A. B. S., and B.H. wrote the manuscript with feedback from all authors.

We thank members of the Haider and Saleem labs and the anonymous reviewers for constructive feedback. This work was supported by the Goizueta Foundation Fellowship (to J.D.R.), Alfred P. Sloan Foundation’s Minority Ph.D. (MPHD) Program Fellowship (to J.D.R.), the Whitehall Foundation (to B.H.), the Alfred P. Sloan Foundation Fellowship In Neuroscience (to B.H.), National Institutes of Health Neurological Disorders and Stroke (NS107968 to B.H.), National Institutes of Health BRAIN Initiative (NS109978 to B.H.), by a grant from the Simons Foundation (SFARI 600343, B.H.), Sir Henry Dale Fellowship from the Wellcome Trust and Royal Society (200501 to A.B.S), Human Science Frontiers Program grant (RGY0076/2018 to A.B.S.).

The authors declare no competing interests.



  • Science & Technology
  • Life Sciences & Biomedicine
  • Biochemistry & Molecular Biology
  • Biology
  • Cell Biology
  • Life Sciences & Biomedicine - Other Topics

Spatial modulation of dark versus bright stimulus in the mouse visual system


Journal Title:



Volume 31, Number 18


, Pages 4172-4179.e6

Type of Work:

Article | Post-print: After Peer Review


A fundamental task of the visual system is to respond to both increases and decreases of luminance with action potentials (ON and OFF responses1–4). OFF responses are stronger, faster, and more salient than ON responses in primary visual cortex (V1) of both cats5,6 and primates,7,8 but in ferrets9 and mice,10 ON responses can be stronger, weaker,11 or balanced12 in comparison to OFF responses. These discrepancies could arise from differences in species, experimental techniques, or stimulus properties, particularly retinotopic location in the visual field, as has been speculated;9 however, the role of retinotopy for ON/OFF dominance has not been systematically tested across multiple scales of neural activity within species. Here, we measured OFF versus ON responses across large portions of visual space with silicon probe and whole-cell patch-clamp recordings in mouse V1 and lateral geniculate nucleus (LGN). We found that OFF responses dominated in the central visual field, whereas ON and OFF responses were more balanced in the periphery. These findings were consistent across local field potential (LFP), spikes, and subthreshold membrane potential in V1, and were aligned with spatial biases in ON and OFF responses in LGN. Our findings reveal that retinotopy may provide a common organizing principle for spatial modulation of OFF versus ON processing in mammalian visual systems.

Copyright information:

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
Export to EndNote