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Author Notes:

Peng Xi, Email, xipeng@pku.edu.cn

The authors declare no competing interests.

Subject:

Keywords:

  • Science & Technology
  • Physical Sciences
  • Optics
  • LIMIT

Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

Tools:

Journal Title:

LIGHT-SCIENCE & APPLICATIONS

Volume:

Volume 11, Number 1

Publisher:

, Pages 293-293

Type of Work:

Article | Final Publisher PDF

Abstract:

A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice. Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process. Consisting of hollow zero-intensity excitation, single-pixel detection, time-correlated single photon counting, and drift stabilization, the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally. An averaged high-SNR reference, and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation. With performance reaching nearly conventional MINFLUX’s, the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.

Copyright information:

© The Author(s) 2022

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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