Design, antihuman immunodeficiency activity and molecular docking studies of synthesized 2-aryl and 2-pyrimidinyl pyrrolidines

• Authors: Sahak P Gasparyan, National Academy of Sciences of RA; Ashot H Martirosyan, National Academy of Sciences of RA; Marina V Alexanyan, National Academy of Sciences of RA; Gohar K Harutyunyan, National Academy of Sciences of RA; Garri V Chilingaryan, Russian Armenian Nivers; Steven Coats, Emory University; Raymond Schinazi, Emory University
• Journal Title: MOLECULAR DIVERSITY
• Volume: Volume 25, Number 4
• Publisher: SPRINGER | 2020-05-05, Pages 2045-2052
• Type of Work: Article | Post-print: After Peer Review
• Publisher DOI: http://doi.org/10.1007/s11030-020-10095-1
• Subjects: Health Sciences, Pharmacology
• Keywords: Science & Technology; Life Sciences & Biomedicine; Physical Sciences; Biochemistry & Molecular Biology; Chemistry, Applied; Chemistry, Medicinal; Chemistry, Multidisciplinary; Chemistry; Pharmacology & Pharmacy; 2-Aryl pyrrolidines; Pyrimidinyl pyrrolidines; Docking studies; Binding energy; Anti-HIV-1 activity; DERIVATIVES; POTENT

Abstract

Abstract: A series of thirty-one new compounds were synthesized and evaluated for their anti-HIV-1 and cytotoxicity activity. Of these, twelve were found to be inhibitors of HIV replications in primary human lymphocytes with median effective concentration (EC50) values < 20 µM. However, most of the compounds demonstrated cytotoxicity in different cells. Our structure activity relationship study identified different patterns. In the series of 2-aryl pyrrolidines, comparing the activity of the compounds containing 2-aryl substituents we observed that compounds 1c, 1f–j, 2f,g with benzyloxyphenyl and isopropoxy groups were more potent. Compounds 1g–j, 2f,g, in which the 1-aryl moiety contained a methyl group in 3,5- or 4-positions also showed high activity. In the series of compounds containing the amide, aminomethyl and nitrile groups we observed an increase in activity with C(O)NH2 < CH2NH2 < CN. In the series of 2-pyrimidinyl pyrrolidines, the best results were demonstrated with derivatives 5e and 5f, in which the presence of a benzyl fragment in 1st and aniline fragment in 6th positions of pyrimidine ring we observed an increase in anti-HIV activity. Molecular docking studies of synthesized compounds with HIV-1 reverse transcriptase enzyme were performed. Binding energies of ligands were estimated, and the interacting amino acids of HIV-1 reverse transcriptase protein were shown. Based on corroborative results of the molecular docking studies and in vitro experiments, we suggest that three groups of synthesized ligands (1c, 1f–i), (2f,g), (5e,f, 7) are of high interest for further research on new drugs against HIV. Graphic abstract: General structure of synthesized 2-aryl and 2-pyrimidinyl pyrrolidines.[Figure not available: see fulltext.].