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Author Notes:

Nono@insti.kitasato-u.ac.jp. Tel.: +81-48-593-1212

Conceptualization, Y.Y. and H.N.; methodology, Y.I. and T.F.; software, Y.S. (Yoshitaka Shimada) and H.N.; validation, Y.I., J.M.S., Y.Y. and K.K.; formal analysis, Y.Y. and H.N.; investigation, Y.Y., H.I., T.O. and H.N.; resources, Y.Y. and T.O.; data curation, T.Y., T.U., N.K. and Y.N.; writing—original draft preparation, H.N.; writing—review and editing, Y.Y. and J.M.S.; visualization, Y.Y. and Y.S. (Yuichi Sato); supervision, M.M., J.M.S. and K.K.; project administration, H.N.; funding acquisition, Y.Y., Y.I., T.F. and H.N. All authors have read and agreed to the published version of the manuscript.

The authors declare no conflict of interest.

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Research Funding:

This research was funded by a Grant-in Aid for Scientific Research from the Ministry of Education, Culture, Sports, Sciences and Technology of Japan (16K09654 (H.N.), 16K08505 (Y.Y.), 18K08247 (Y.I.), and 19K09226 (T.F.)) and by a grant from the Kitasato University Medical Center (H25–003 (T.F.) and H24–011 (H.N.)).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Physical Sciences
  • Biochemistry & Molecular Biology
  • Chemistry, Multidisciplinary
  • Chemistry
  • erythropoietin
  • angiotensin II
  • renin-angiotensin-aldosterone system
  • deglycosylation
  • Western blotting
  • HIF2 alpha
  • proximal tubules
  • collecting ducts
  • EXPRESSION
  • ANEMIA
  • URINE

Effects of Angiotensin II on Erythropoietin Production in the Kidney and Liver

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Journal Title:

MOLECULES

Volume:

Volume 26, Number 17

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Type of Work:

Article | Final Publisher PDF

Abstract:

The kidney is a main site of erythropoietin production in the body. We developed a new method for the detection of Epo protein by deglycosylation-coupled Western blotting. Detection of deglycosylated Epo enables the examination of small changes in Epo production. Using this method, we investigated the effects of angiotensin II (ATII) on Epo production in the kidney. ATII stimulated the plasma Epo concentration; Epo, HIF2α, and PHD2 mRNA expression in nephron segments in the renal cortex and outer medulla; and Epo protein expression in the renal cortex. In situ hybridization and immunohistochemistry revealed that ATII stimulates Epo mRNA and protein expression not only in proximal tubules but also in collecting ducts, especially in intercalated cells. These data support the regulation of Epo production in the kidney by the renin–angiotensin– aldosterone system (RAS).

Copyright information:

© 2021 by the authors.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/rdf).
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