Drosophila centrocortin is dispensable for centriole duplication but contributes to centrosome separation

• Authors: Dipen S Mehta, Augusta University; Hala Zein-Sabatto, Emory University; Pearl Ryder, Emory University; Jina Lee, Emory University; Dorothy Lerit, Emory University
• Journal Title: G3-GENES GENOMES GENETICS
• Volume: Volume 12, Number 2
• Publisher: OXFORD UNIV PRESS INC | 2022-02-04
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1093/g3journal/jkab434
• Keywords: Science & Technology; Life Sciences & Biomedicine; Genetics & Heredity; centrosome; mitosis; RNA localization; centrocortin; MESSENGER-RNAS; STEM-CELL; LOCALIZATION; ORGANIZATION; INHERITANCE; REVEALS

Abstract

Centrosomes are microtubule-organizing centers that duplicate exactly once to organize the bipolar mitotic spindle required for error-free mitosis. Prior work indicated that Drosophila centrocortin (cen) is required for normal centrosome separation, although a role in centriole duplication was not closely examined. Through time-lapse recordings of rapid syncytial divisions, we monitored centriole duplication and the kinetics of centrosome separation in control vs cen null embryos. Our data suggest that although cen is dispensable for centriole duplication, it contributes to centrosome separation.