Interactions Between Commensal Bacteria and Enteric Neurons, via FPR1 Induction of ROS, Increase Gastrointestinal Motility in Mice

• Authors: Bindu Chandrasekharan, Emory University; Bejan J Saeedi, Emory University; M. Ashfaqul Alam, Emory University; Madelyn Houser, Emory University; Shanthi Srinivasan, Emory University; MariadeLourdes Tansey, Emory University; Rheinallt Jones, Emory University; Asma Nusrat, Emory University; Andrew Neish, Emory University
• Journal Title: GASTROENTEROLOGY
• Volume: Volume 157, Number 1
• Publisher: W B SAUNDERS CO-ELSEVIER INC | 2019-07-01, Pages 179-+
• Type of Work: Article | Post-print: After Peer Review
• Publisher DOI: http://doi.org/10.1053/j.gastro.2019.03.045
• Subjects: Health Sciences, Pathology; Health Sciences, Medicine and Surgery
• Keywords: Science & Technology; Life Sciences & Biomedicine; Gastroenterology & Hepatology; Microbiome; Signal Transduction; ERK; Digestion; FORMYL PEPTIDE RECEPTOR; LACTOBACILLUS-RHAMNOSUS GG; EXCITABILITY; DYSFUNCTION; RESTITUTION; EXPRESSION; GENERATION; SEROTONIN; BEHAVIOR; REUTERI

Abstract

Background & Aims: Reduced gastrointestinal (GI) motility is a feature of disorders associated with intestinal dysbiosis and loss of beneficial microbes. It is not clear how consumption of beneficial commensal microbes, marketed as probiotics, affects the enteric nervous system (ENS). We studied the effects of the widely used probiotic and the commensal Lactobacillus rhamnosus GG (LGG) on ENS and GI motility in mice. Methods: Conventional and germ free C57B6 mice were gavaged with LGG and intestinal tissues were collected; changes in the enteric neuronal subtypes were assessed by real-time polymerase chain reaction, immunoblots, and immunostaining. Production of reactive oxygen species (ROS) in the jejunal myenteric plexi and phosphorylation (p) of mitogen-activated protein kinase 1 (MAPK1) in the enteric ganglia were assessed by immunoblots and immunostaining. Fluorescence in situ hybridization was performed on jejunal cryosections with probes to detect formyl peptide receptor 1 (FPR1). GI motility in conventional mice was assessed after daily gavage of LGG for 1 week. Results: Feeding of LGG to mice stimulated myenteric production of ROS, increased levels of phosphorylated MAPK1, and increased expression of choline acetyl transferase by neurons (P <.001). These effects were not observed in mice given N-acetyl cysteine (a ROS inhibitor) or LGGΩSpaC (an adhesion-mutant strain of LGG) or FPR1-knockout mice. Gavage of mice with LGG for 1 week significantly increased stool frequency, reduced total GI transit time, and increased contractions of ileal circular muscle strips in ex vivo experiments (P <.05). Conclusions: Using mouse models, we found that LGG-mediated signaling in the ENS requires bacterial adhesion, redox mechanisms, and FPR1. This pathway might be activated to increase GI motility in patients.