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Gowen Cook, jean_cook@med.unc.edu
J.P. Matson designed and performed the experiments and analyzed the results. A.M. House tracked cells from live cell imaging and processed data. G.D. Grant tracked cells and ran the microscope. H. Wu performed some immunoblots. J. Perez created the RPE1 Cdc6-mVenus, PCNA-mTurq2, DHB-mCherry cell line. J.G. Cook designed experiments and supervised the project. J.P. Matson and J.G. Cook wrote the manuscript with input from the other authors.
We thank Jeffrey Jones for managerial assistance and the Cook laboratory for helpful discussion. We thank Dr. Sam Wolff, Seraphina Wong, Aimee Littlejohn, and Walli Driggers for support. We thank Dr. Kasia Kedziora for help with imaging analysis. The DHB-mCherry plasmid was a gift from Dr. Sabrina Spencer. The Cdc6-mVenus plasmid was a gift from Dr. Michael Brandeis. The RPE1_hTert p53 CRIPSR null line was a gift from Dr. Prasad Jallepalli.
The authors declare no competing financial interests.
This work was supported by a fellowship from the National Science Foundation (DGE-1144081) to J.P. Matson, a University of North Carolina Dissertation Completion Fellowship to J.P. Matson, and grants from the National Institutes of Health/National Institute of General Medical Sciences to J.G. Cook (GM083024, GM102413, and R25GM089569; R25GM089569 also supported J. Perez). The University of North Carolina Flow Cytometry Core Facility is supported in part by P30 CA016086.
© 2019 Matson et al.