Dual-modality optical coherence tomography and fluorescence tethered capsule endomicroscopy

• Authors: Andreas Wartak, Massachusetts General Hospital; Alfred K Kelada, Massachusetts General Hospital; Paola AL Alarcon, Massachusetts General Hospital; Ara L Bablouzian, Massachusetts General Hospital; Osman O Ahsen, Massachusetts General Hospital; Abigail L Gregg, Massachusetts General Hospital; Yuxiao Wei, Massachusetts General Hospital; Keval Bollavaram, Emory University; Conor J Sheil, Massachusetts General Hospital; Edward Farewell, Massachusetts General Hospital; Schuyler VanTol, Massachusetts General Hospital; Rachel Smith, Massachusetts General Hospital; Patricia Grahmann, Massachusetts General Hospital; Aaron R Baillargeon, Massachusetts General Hospital; Joseph A Gardecki, Massachusetts General Hospital; Guillermo J Tearney, Massachusetts General Hospital
• Journal Title: BIOMEDICAL OPTICS EXPRESS
• Volume: Volume 12, Number 7
• Publisher: OPTICAL SOC AMER | 2021-07-01, Pages 4308-4323
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1364/BOE.422453
• Subjects: Health Sciences, Pathology; Engineering, Biomedical
• Keywords: Science & Technology; Life Sciences & Biomedicine; Physical Sciences; Biochemical Research Methods; Optics; Radiology, Nuclear Medicine & Medical Imaging; Biochemistry & Molecular Biology; BARRETTS-ESOPHAGUS; INTESTINAL METAPLASIA; IN-VIVO; CORONARY ATHEROSCLEROSIS; GASTROINTESTINAL-TRACT; EX-VIVO; OCT; IDENTIFICATION; ENDOSCOPY; DYSPLASIA

Abstract

OCT tethered capsule endomicroscopy (TCE) is an emerging noninvasive diagnostic imaging technology for gastrointestinal (GI) tract disorders. OCT measures tissue reflectivity that provides morphologic image contrast, and thus is incapable of ascertaining molecular information that can be useful for improving diagnostic accuracy. Here, we introduce an extension to OCT TCE that includes a fluorescence (FL) imaging channel for attaining complementary, co-registered molecular contrast. We present the development of an OCT-FL TCE capsule and a portable, plug-and-play OCT-FL imaging system. The technology is validated in phantom experiments and feasibility is demonstrated in a methylene blue (MB)-stained swine esophageal injury model, ex vivo and in vivo.