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Qihui Wang, wangqihui@im.ac.cn; George F. Gao, gaof@im.ac.cn; Jianxun Qi, jxqi@im.ac.cn
J.Q., G.F.G., and Q.W. initiated and designed the project. K.L. and X.P. purified the proteins and grew the crystals with help from Y.M., Y.Z., L.W., and C.S. X.P. and L.L. performed the SPR analysis with help from Y.J., S.N., and C.Q. K.L., X.P., and L.L. conducted the flow cytometry assay with help from A.Z., Q.C., and L.W. F.Y. and J.Q. collected the structural data and solved the structures. K.L., F.Y., S.T., and J.Q. analyzed the data and prepared the figures with help from D.M. and X.M. A.Z., P.H., and X.Z. performed pseudovirus-related assays. D.L. and Z.C. collected the serum samples. K.L., P.D., Q.W., and G.F.G. wrote and revised the manuscript.
We are grateful to William Ningda Gao for revising the manuscript and Hillary Yida Zhang for constructing the RaTG13 6-mutant plasmid. We are grateful to the Pathogenic Microbiology and Immunology Public Technology Service Center for its support regarding the flow cytometry assay. We acknowledge the staff of beamline BL19U1 at the Shanghai Synchrotron Radiation Facility for assistance during data collection. We also thank Y. Chen, B. Zhou, and Z. Yang from the Institute of Biophysics, Chinese Academy of Sciences, for technical support with the SPR assay. We acknowledge Z. Shi from Wuhan Institute of Virology, Chinese Academy of Sciences, for providing the ACE2 sequence of Rhinolophus affinis.
The authors declare no competing interests.
This work was supported by the Ministry of Science and Technology of the People's Republic of China; the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB29010202, XDB29040203, and XDB37030204); an intramural special grant for SARS-CoV-2 research from the Chinese Academy of Sciences, National Natural Science Foundation of China (81922044), and the National Science and Technology Major Project (2018ZX10101004-001 and 2018ZX09711003-002-001). Q.W. is supported by the Youth Innovation Promotion Association of the CAS (2018119). G.F.G is supported by the Yanqi Lake Meeting organized by the academic divisions of the CAS.
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