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Author Notes:

andrea.moreno@uc.cl

Conceptualization, D.R.; Formal analysis, R.B.-M.; Funding acquisition, A.I.M.-S.; Investigation, F.D., D.R. and S.E.; Methodology, R.B.-M., R.G., S.E. and A.I.M.-S.; Project administration, A.I.M.-S.; Supervision, A.O.-C. and A.I.M.-S.; Visualization, R.B.-M.; Writing—original draft, R.B.-M. and R.G.; Writing—review and editing, D.R., A.O.-C., S.E. and A.I.M.-S. All authors have read and agreed to the published version of the manuscript.

The authors declare that there are no conflicts of interest.

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Research Funding:

We acknowledge the funding sources: 1 [ANID FONDECYT 1181167 to AIM-S], 2 [ANID Millennium Science Initiative/Millennium Initiative for Collaborative Research on Bacterial Resistance NCN17_081 to AIMS]. Genome sequencing was provided by MicrobesNG (http://www.microbesng.uk, accessed on 1 September 2017), which is supported by the BBSRC (BB/L024209/1). Rodrigo García thanks CONICYT FECHA DOCTORADO/2016 21161133.

Keywords:

  • bacteriophages
  • genomes
  • Felixounavirus
  • Salmonella spp.
  • Salmonella phages
  • comparative genomics

Comparative Analysis of Felixounavirus Genomes Including Two New Members of the Genus That Infect Salmonella Infantis

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Journal Title:

Antibiotics

Volume:

Volume 10, Number 7

Publisher:

Type of Work:

Article | Final Publisher PDF

Abstract:

Salmonella spp. is one of the most common foodborne pathogens worldwide; therefore, its control is highly relevant for the food industry. Phages of the Felixounavirus genus have the characteristic that one phage can infect a large number of different Salmonella serovars and, thus, are proposed as an alternative to antimicrobials in food production. Here, we describe two new members of the Felixounavirus genus named vB_Si_35FD and vB_Si_DR94, which can infect Salmonella Infantis. These new members were isolated and sequenced, and a subsequent comparative genomic analysis was conducted including 23 publicly available genomes of Felixounaviruses that infect Salmonella. The genomes of vB_Si_35FD and vB_Si_DR94 are 85,818 and 85,730 bp large and contain 129 and 125 coding sequences, respectively. The genomes did not show genes associated with virulence or antimicrobial resistance, which could be useful for candidates to use as biocontrol agents. Comparative genomics revealed that closely related Felixounavirus are found in distinct geographical locations and that this genus has a conserved genomic structure despite its worldwide distribution. Our study revealed a highly conserved structure of the phage genomes, and the two newly described phages could represent promising biocontrol candidates against Salmonella spp. from a genomic viewpoint.

Copyright information:

© 2021 by the authors.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/rdf).
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