In-vivo diffusion MRI protocol optimization for the chimpanzee brain and examination of aging effects on the primate optic nerve at 3T

• Authors: Chunxia Li, Emory University; James Rilling, Emory University; Xiaoping Hu, Emory University; Todd Preuss, Emory University; Xiaodong Zhang, Emory University; Longchuan Li, Emory University; James Herndon Jr., Emory University; Y Yan, Emory University; G Nair, NINDS; JG Herndon, Emory University
• Journal Title: MAGNETIC RESONANCE IMAGING
• Volume: Volume 77, Number
• Publisher: ELSEVIER SCIENCE INC | 2021-01-13, Pages 194-203
• Type of Work: Article
• Publisher DOI: http://doi.org/10.1016/j.mri.2020.12.015
• Keywords: Science & Technology; Life Sciences & Biomedicine; Radiology, Nuclear Medicine & Medical Imaging; DTI; Fiber tracking; Distortion correction; Optic nerve; Non-human primate; Large animals; Aging; WHITE-MATTER INTEGRITY; CEREBRAL-BLOOD-FLOW; AGE-RELATED DECLINE; ECHO-PLANAR IMAGES; NONHUMAN-PRIMATES; RHESUS-MONKEY; RADIAL DIFFUSIVITY; COGNITIVE DECLINE; IMAGING DETECTS; TENSOR

Abstract

Background: Diffusion MRI (dMRI) data acquisition protocols are well-established on modern high-field clinical scanners for human studies. However, these protocols are not suitable for the chimpanzee (or other large-brained mammals) because of its substantial difference in head geometry and brain volume compared with humans. Therefore, an optimal dMRI data acquisition protocol dedicated to chimpanzee neuroimaging is needed. Methods: A multi-shot (4 segments) double spin-echo echo-planar imaging (MS-EPI) sequence and a single-shot double spin-echo EPI (SS-EPI) sequence were optimized separately for in vivo dMRI data acquisition of chimpanzees using a clinical 3T scanner. Correction for severe susceptibility-induced image distortion and signal drop-off of the chimpanzee brain was performed and evaluated using FSL software. DTI indices in different brain regions and probabilistic tractography were compared. A separate DTI data set from n=34 chimpanzees (13 to 56 years old) was collected using the optimal protocol. Age-related changes in diffusivity indices of optic nerve fibers were evaluated. Results: The SS-EPI sequence acquired dMRI data of the chimpanzee brain with approximately doubled the SNR as the MS-EPI sequence given the same scan time. The quality of white matter fiber tracking from the SS-EPI data was much higher than that from MS-EPI data. However, quantitative analysis of DTI indices showed no difference in most ROIs between the SS-EPI and MS-EPI sequences. The progressive evolution of diffusivity indices of optic nerves indicated mild changes in fiber bundles of chimpanzees aged 40 years and above. Conclusion: The single-shot EPI-based acquisition protocol provided better image quality of dMRI for chimpanzee brains and is recommended for in vivo dMRI study or clinical diagnosis of chimpanzees (or other large animals) using a clinical scanner. Also, the tendency of FA decrease or diffusivity increase in the optic nerve of aged chimpanzees was seen but did not show significant age-related changes, suggesting aging may have less impact on optic nerve fiber integrity of chimpanzees, in contrast to previous results for both macaque monkeys and humans.