89 Views | 53 Downloads
X. Frank Zhang, Email: xiz310@lehigh.edu
N.A.A., E.R.L., and M.S.W. cloned and generated VWF fragments; N.A.A. performed BLI experiments, produced, and ran experiments with VHH81 and 6G1; W.C. performed single-molecule experiments; N.A.A. and E.R.L. ran platelet aggregometry experiments; A.K.B., E.X., and J.E. crystallized and refined structures; M.C.B. provided critical reagents; X.F.Z. and R.L. designed the research; N.A.A., W.C., X.F.Z., J.E., and R.L. analyzed the results; N.A.A., W.C., J.E., X.F.Z., and R.L. prepared the figures; N.A.A. and R.L. wrote the paper; all authors edited the paper.
The authors would like to thank Diamond Light Source for beamtime (proposal MX19880), and the staff of beamlines I04 for assistance.
The authors declare no competing interests.
This work was supported in part by NIH research grants (HL082808, HL143794, and HL152348), NIH training grants (GM008602 and GM008367), a studentship grant from the British Heart Foundation (FS/18/70/33893) awarded to J.E./A.K.B., and an infrastructure grant from Hemophilia of Georgia Center for Bleeding & Clotting Disorders of Emory. N.A.A. was supported in part by AHA grant 20PRE34990025 and NIH fellowship HL154656. E.R.L. was supported in part by NIH fellowship HL149357.
© The Author(s) 2021