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pselvar@emory.edu

Conceptualization: P.S., C.D.P., R.B., S.R., M.K., S.-M.K. and M.S.S.; Methodology: C.D.P., S.R., P.S., R.B., M.K., S.-M.K., K.M.J., L.J., S.-H.N., A.N.B., T.V.d.G., C.N.W. and L.L.; Investigation: R.B., L.E.M., J.O., K.-H.K., N.B., S.S., P.K. and J.T.L.B.; Funding acquisition: P.S., S.J.C.R., C.D.P. and S.-M.K.; Project administration: C.D.P., S.R., P.S., S.-M.K., M.K. and M.S.S.; Supervision: R.B., S.-M.K., P.S., S.R., C.D.P., M.S.S. and M.K.; Writing—original draft: R.B., S.-M.K., M.K., C.D.P. and S.R.; Writing—review and editing: P.S., S.-M.K., M.K. and M.S.S. All authors have read and agreed to the published version of the manuscript.

P.S. and S.J.C.R. are the co-founders of the Metaclipse Therapeutics Corporation (MTC) and hold equity and stock options. The corresponding author (P.S.) holds shares in Metaclipse Therapeutics Corporation, a company that is planning to use GPI-anchored molecules to develop a VLP-based vaccine in the future, as suggested in the current manuscript. C.D.P. and S.R. declare competing financial interests in the form of stock ownership and paid employment by Metaclipse Therapeutics Corporation. K.M.J., S.-H.N., A.N.B., L.J., T.V.G. and C.N.W. declare competing financial interests in the form of paid employment by Metaclipse Therapeutics Corporation. One or more embodiments of one or more patents and patent applications filed by Metaclipse Therapeutics Corporation and Emory University may encompass the methods, reagents, and data disclosed in this manuscript. All other authors have no competing interests to declare.

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Research Funding:

This research was funded by NIH/NIAID (SBIR Contract# 75N93019C00017 Amendment to Pack/Ramachandiran) and Intel Corporation for the Intel COVID-19 Global Technology Response Initiative grant. The animal study protocols were approved by the Institutional Animal Care and Use Committee (IACUC). IACUC protocol # 2017-00-504 (Emory University), Protocol number A20044 (Georgia State University).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Immunology
  • Medicine, Research & Experimental
  • Research & Experimental Medicine
  • influenza
  • virus-like particles
  • SARS-CoV-2
  • GM-CSF
  • RBD
  • mice
  • IL-12
  • antibodies
  • COLONY-STIMULATING FACTOR
  • TUMOR-ASSOCIATED ANTIGENS
  • DENDRITIC CELLS
  • PROTEIN TRANSFER
  • SIPULEUCEL-T
  • INTERLEUKIN-12
  • IMMUNOTHERAPY
  • IMMUNOGENICITY
  • ADJUVANTS

Influenza Virus-like Particle-Based Hybrid Vaccine Containing RBD Induces Immunity against Influenza and SARS-CoV-2 Viruses

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Journal Title:

VACCINES

Volume:

Volume 10, Number 6

Publisher:

Type of Work:

Article | Final Publisher PDF

Abstract:

Several approaches have produced an effective vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since millions of people are exposed to influenza virus and SARS-CoV-2, it is of great interest to develop a two-in-one vaccine that will be able to protect against infection of both viruses. We have developed a hybrid vaccine for SARS-CoV-2 and influenza viruses using influenza virus-like particles (VLP) incorporated by protein transfer with glycosylphosphatidylinositol (GPI)-anchored SARS-CoV-2 RBD fused to GM-CSF as an adjuvant. GPI-RBD-GM-CSF fusion protein was expressed in CHO-S cells, purified and incorporated onto influenza VLPs to develop the hybrid vaccine. Our results show that the hybrid vaccine induced a strong antibody response and protected mice from both influenza virus and mouse-adapted SARS-CoV-2 challenges, with vaccinated mice having significantly lower lung viral titers compared to naive mice. These results suggest that a hybrid vaccine strategy is a promising approach for developing multivalent vaccines to prevent influenza A and SARS-CoV-2 infections.

Copyright information:

© 2022 by the authors.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/rdf).
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