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John R. Hepler, jhepler@emory.edu
K. E. S. and J. R. H. conceptualization; K. E. S., K. J. G., M. C. T., D. J. L., C. M. M., M. Z., S. N. R., C. D. S., R. N. S., F. J. S., and J. P. S. data curation; E. A. O., D. W., S. M. D., and J. R. H. funding acquisition; K. E. S. validation; K. E. S. investigation; K. E. S., M. C. T., D. J. L., S. R., and C. D. S. visualization; K. E. S., K. J. G., M. C. T., D. J. L., M. Z., C. D. S., and J. P. S. methodology; K. E. S. writing original draft; K. E. S. and J. R. H. writing - reviewing and editing; E. A. O., D. W., S. M. D., and J. R. H. resources; J. R. H. and S. M. D. supervision; J. R. H. and S. M. D. project administration.
The authors would like to acknowledge and thank Dr YuhMin Chook for generously providing the CRM1 (XPO1) cDNA construct and guidance on its interaction conditions, Dr Jeremy Boss for constructive input and contributions regarding RNA sequencing, and the Emory Neurosciences—NINDS Core Facilities (ENNCF) for assistance with imaging and histological staining. The authors also thank the NIEHS Viral Vector Core and the NIEHS Fluorescence Microscopy and Imaging Center. Thanks also go the Emory Transgenic Mouse and Gene Targeting Core for assistance with generating the CRISPR/Cas9 gene editing mice.
The authors declare that they have no conflicts of interest with the contents of this article.
This research was supported by funding from the National Institutes of Health awards 2R21NS102652 (to J. R. H.) and R01NS037112 (to J. R. H.), the Intramural Research Program of the National Institute of Environmental Health Sciences, National Institutes of Health (ES 100221 to S. D.), and the Neuropathology/Histochemistry Core of the Emory NINDS Neurosciences Core Facility (P30 NS055077). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
© 2020 The Authors