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Correspondence: Rick L. Tarleton, Center for Tropical and Emerging Global Diseases, Coverdell Center for Biomedical Research, 500 D.W. Brooks Dr, University of Georgia, Athens, GA 30602, (706) 542-3362, tarleton@uga.edu
Author contributions: HS designed the studies, conducted experiments and acquired and analyzed data. DC conducted experiments and provided reagents, DF, KWM, SAH, SMT, NR, SE and TMR provided reagents and edited the manuscript. LW provided reagents. MHC helped design the studies, provided reagents and edited the manuscript. RLT designed the studies, analyzed the data and wrote the manuscript.
Acknowledgements: We thank the participants who provided blood or sputum specimens that were tested in this study. We thank the faculty and staff team of The Hope Clinic for a dedicated effort in recruiting subjects and collecting specimens and Hannah Hanley and Mitchell Lee for the processing of the serum and saliva samples.
We thank Dr. Yerun Zhu and Dr. Daniel Espinoza for assistance with laboratory operations. We thank Italo Zecca, Lisa Auckland, Edward Davila, Gabriel Hamer, Chris Roundy, and Wendy Tang for collecting samples from pets. Mia Torchetti, Melinda Jenkins Moore, and Katie Mozingo provided virus neutralization testing of animal samples.
Plasmids for expression of SARS-CoV-2 Spike and RBD proteins, as well as CR3022 monoclonal antibody were generously provided by Dr. Florian Krammer (Icahn School of Medicine at Mount Sinai, produced under NIAID CEIRS contract HHSN272201400008C).
Disclosures: The authors have declared that no conflicts of interest exists.
Financial support for this work was provided by grant R01AI125738 to RLT from the National Institutes of Health and a University of Georgia Athletic Association endowment to RLT. S.M.T. and T.M.R. are partially supported by NIAID Centers of Excellence for Influenza Research and Surveillance (CEIRS) contract HHSN272201400004C and from the University of Georgia.
T.M.R and LW are also supported, in part, by the Georgia Research Alliance as an Eminent Scholar and a Distinguished Investigator, respectively. Additional support for specimen collection and processing of samples in cohort 2 was provided by the Georgia Emerging Infections Program, which was funded through the Centers for Disease Control and Prevention Emerging Infections Program [U50CK000485].
Pet dog and cat samples were collected with funding from the Centers for Disease Control and Prevention RFP 75D 301-20-R-68167.
The copyright holder for this preprint is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.