Host plant diet affects growth and induces altered gene expression and microbiome composition in the wood white (Leptidea sinapis) butterfly

• Authors: Karin Nasvall, Uppsala University; Christer Wiklund, Stockholm University; Veronika Mrazek, Uppsala University; Axel Kunstner, University of Lubeck; Venkat Talla, Emory University; Hauke Busch, University of Lubeck; Roger Vila, Institut de Biologia Evolutiva (CSIC‐UPF), Barcelona Spain; Niclas Backstrom, Uppsala University
• Journal Title: Molecular Ecology
• Volume: Volume 30, Number 2
• Publisher: Wiey | 2021-01-01, Pages 499-516
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1111/mec.15745
• Subjects: Biology, General; Biology, Genetics; Biology, Ecology
• Keywords: gene expression; host plant; Lepidoptera; microbiome; speciation; wood white

Abstract

In a time with decreasing biodiversity, especially among insects, a detailed understanding about specific resource utilization strategies is crucial. The physiological and behavioural responses to host switches in phytophagous insects are poorly understood. Earlier studies indicate that a host plant switch might be associated with distinctive molecular and physiological responses in different lineages. Expanding the assessment of such associations across Lepidoptera will reveal if there are general patterns in adaptive responses, or if each switch event is more of a unique character. We investigated host plant preference, fitness consequences, effects on expression profiles and gut microbiome composition in two common wood white (Leptidea sinapis) populations with different host plant preferences from the extremes of the species distribution area (Sweden and Catalonia). Our results show that female Catalonian wood whites lack preference for either host plant (Lotus corniculatus or L. dorycnium), while Swedish females laid significantly more eggs on L. corniculatus. Individuals from both populations reared on L. dorycnium had longer developmental times and smaller body size as adults. This indicates that both environmental and genetic factors determine the choice to use a specific host plant. Gene expression analysis revealed a more pronounced response to host plant in the Catalonian compared to the Swedish population. In addition, host plant treatment resulted in a significant shift in microbiome community structure in the Catalonian population. Together, this suggests that population specific plasticity associated with local conditions underlies host plant utilisation in wood whites.