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Author Notes:


S. K. and H. G. conceptualization; S. K., R. E. D., T. F. R. H., and H. G. data curation; S. K., R. E. D., and H. G. formal analysis; S. K., R. E. D., and H. G. validation; S. K., R. E. D., T. F. R. H., and H. G. investigation; S. K., R. E. D., and H. G. visualization; S. K. and H. G. methodology; S. K. and H. G. writing-original draft; S. K. and H. G. project administration; S. K. and H. G. writing-review and editing; H. G. resources; H. G. supervision; H. G. funding acquisition.

We thank J. A. Ball for purification of Nop581–437 and GST-Snu13, and K. Araki for help with gradient fractionation. We also thank K. P. Hopfner for the Rvb1/2 expression plasmid; W. Houry and K. Karbstein for antibodies; and G. L. Conn, C. M. Dunham, D. Reines, and members of the Ghalei lab for comments on the manuscript.



  • Science & Technology
  • Life Sciences & Biomedicine
  • Biochemistry & Molecular Biology
  • ribosome assembly
  • RNA
  • RNA binding protein
  • RNA modification
  • RNA methylation
  • small nucleolar RNA (snoRNA)
  • ribonuclear protein (RNP)
  • assembly factor
  • box C
  • D snoRNA
  • snoRNP assembly
  • Bcd1
  • Snu13
  • small nucleolar ribonucleoprotein (snoRNP) assembly
  • zinc finger HIT-type containing 6 (ZNHIT6)
  • ribosome biogenesis
  • posttranscriptional regulation
  • rRNA processing
  • HSP90
  • RVB1/RVB2
  • 15.5K

A conserved Bcd1 interaction essential for box C/D snoRNP biogenesis


Journal Title:



Volume 294, Number 48


, Pages 18360-18371

Type of Work:

Article | Final Publisher PDF


Precise modification and processing of rRNAs are required for the production of ribosomes and accurate translation of proteins. Small nucleolar ribonucleoproteins (snoRNPs) guide the folding, modification, and processing of rRNAs and are thus critical for all eukaryotic cells. Bcd1, an essential zinc finger HIT protein functionally conserved in eukaryotes, has been implicated as an early regulator for biogenesis of box C/D snoRNPs and controls steady-state levels of box C/D snoRNAs through an unknown mechanism. Using a combination of genetic and biochemical approaches, here we found a conserved N-terminal motif in Bcd1 from Saccharomyces cerevisiae that is required for interactions with box C/D snoRNAs and the core snoRNP protein, Snu13. We show that both the Bcd1-snoRNA and Bcd1- Snu13 interactions are critical for snoRNP assembly and ribosome biogenesis. Our results provide mechanistic insight into Bcd1 interactions that likely control the early steps of snoRNP maturation and contribute to the essential role of this protein in maintaining the steady-state levels of snoRNAs in the cell.

Copyright information:

© 2019 Khoshnevis et al.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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