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Correspondence: rbanerje@umich.edu
Author contributions: M.R. and G.C.C. performed the majority of the biochemical studies, H.G. did the kinetic analysis of hMCM, and L.M. and M.R. cloned and expressed the Mtb genes used in this study.
M.P. and M.K. were responsible for the crystallographic analyses, and K.W. was responsible for the EPR spectroscopic analysis. H.S. performed the macrophage experiments, and J.Z. and S.W. performed the Mtb growth experiments. M.R., G.C.C., and R.B. wrote the manuscript.
All authors were involved with data analysis of experiments performed by them or in their laboratories and edited the manuscript.
We acknowledge H. Sharma (University of Michigan) for his assistance with crystallization and the GM/CA CAT at the Advanced Light Source for beam time.
Disclosures: V.K.M. is an Investigator of the Howard Hughes Medical Institute. V.K.M. is a paid advisor to Janssen Pharmaceuticals and 5AM Ventures and owns equity in Raze Therapeutics.
We also acknowledge the NIH Common Fund Metabolite Standards Synthesis Core (NHLBI contract no. HHSN268201300022C) for providing [13C]itaconate.
his work was supported in part by grants from the National Institutes of Health (RO1-DK45776 to R.B., 5 F32 GM113405 to G.C.C., K99-GM124296 to H.S., R35GM122455 to V.K.M., R01-DK054514 to K.W., and U19AI107774 to E.J.R.) and start-up funds from the University of Michigan (to M.K.).
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