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Correspondence: Heather Flanagan-Steet, Greenwood Genetic Center, J.C. Self Research Institute, 113 Gregor Mendel Circle, Greenwood, South Carolina 29646, USA. Phone: 864.388.1806; Email: heatherfs@ggc.org
Author contributions: PL, TM, and CJC performed experiments and interpreted data. TCL provided the DNA constructs containing TALEN sequences used to generate gnptab-KO lines. RAS assisted with data interpretation and manuscript preparation. HFS designed and performed experiments, interpreted data, and wrote the manuscript.
We would like to thank Brian Kwan for his early contributions and help generating the gnptab-KO line. We would also like to thank Matthew Bogyo (Stanford University) for contributing the BMV109 and BMV157 probes used in this study.
We would like to thank Koichi Kawakami and Yoshiko Takahashi (National Institute of Genetics, Japan) for providing the TOL2 transgenesis constructs (MTA K2019-055) (68). Additionally, we are grateful to Andrei Alexandrov (Clemson Center for Human Genetics) for his expert assistance with FAC sorting of zebrafish cells.
We would also like to acknowledge that much of the zebrafish work related to this study was performed on animals raised in the Hazel and Bill Allin Aquaculture Facility housed at the Greenwood Genetic Center. We thank the staff of the facility for their excellent animal care and husbandry.
This work was supported by an R01 to RS from the NIHGMS GM086524, grants to HFS from the National MPS Society and the ISMRD, as well as a grant to RS by the Yash Ghandi Foundation.
Additionally, we have received funds from the National MPS Society and ISMRD.
© 2020, Lu et al.