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Author Notes:

Correspondence: Thierry Hennet, thierry.hennet@uzh.ch

Author contributions: TH designed the study and secured the funding. TR and DB submitted the ethical approval and organized the blood samples. KK performed and analyzed the experiments. YL performed the array experiments. KK and TH wrote the manuscript. TR, DB, DS, and YL revised the manuscript. All authors contributed to the article and approved the submitted version.

We thank the staff, nurses and the laboratory of transfusion medicine (University Hospital Zurich) for helping to organize the supply of blood samples. Parts of the data analysis of array data were performed with support from Lennart Opitz, Functional Genomics Center Zurich, UZH & ETH, Zurich.

Disclosures: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Subjects:

Research Funding:

This work was supported by the Swiss National Foundation grant 314730_172880 and by Novartis FreeNovation to TH and partially supported by the Emory Comprehensive Glycomics Core (ECGC), which is an Emory Integrated Core Facility subsidized by the Emory University School of Medicine.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Immunology
  • immunoglobulin
  • glycosylation
  • glycan
  • microarray
  • microbiota
  • bacteroides
  • Cord blood
  • Anticarbohydrate antibodies
  • Placental transfer
  • Serum antibodies
  • Immunoglobulin G
  • FC receptor
  • IGG
  • Vaccination
  • Transport
  • Responses

Limited Neonatal Carbohydrate-Specific Antibody Repertoire Consecutive to Partial Prenatal Transfer of Maternal Antibodies

Tools:

Journal Title:

Frontiers in Immunology

Volume:

Volume 11

Publisher:

, Pages 573629-573629

Type of Work:

Article | Final Publisher PDF

Abstract:

Despite the prominence of carbohydrate-specific antibodies in human sera, data on their emergence and antigen specificities are limited. Whereas maternal IgG are transferred prenatally to the fetal circulation, IgM present in cord blood originate from fetal B lymphocytes. Considering the limited exposure of the fetus to foreign antigens, we assessed the repertoire of carbohydrate-specific antibodies in human cord blood and matched maternal blood samples using glycan arrays. Carbohydrate-specific IgM was absent in cord blood, whereas low cord blood IgG reactivity to glycans was detectable. Comparing IgG reactivities of matched pairs, we observed a general lack of correlation in the antigen specificity of IgG from cord blood and maternal blood due to a selective exclusion of most carbohydrate-specific IgG from maternofetal transfer. Given the importance of intestinal bacteria in inducing carbohydrate-specific antibodies, we analyzed global antibody specificities toward commensal bacteria. Similar IgG reactivities to specific Bacteroides species were detected in matched cord and maternal blood samples, thus pointing to an efficient maternal transfer of anti-microbial IgG. Due to the observed selectivity in maternofetal IgG transfer, the lack of fetal antibodies to carbohydrate epitopes is only partially compensated by maternal IgG, thus resulting in a weak response to carbohydrate antigens in neonates.

Copyright information:

© 2020 Kappler, Restin, Lasanajak, Smith, Bassler and Hennet.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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