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K.S. and Z.I. conceived the study. Z.I., C.Y., B.A.C., B.D., E.G., D.B., and K.S. analyzed data. Z.I., B.D., H.J., and G.N.J conducted imaging experiments. Z.I., J.S., and C.F. conducted BH3 profiling experiments. C.S., D.J.H., and H.J. provided samples for analysis. L.K. conducted pathological analysis of samples. Z.I. and K.S. wrote the manuscript. All authors edited and approved the final manuscript.
We thank the members of our labs for their comments and suggestions on this work, especially Kaitlyn Webster for assistance with image quantification. We thank Olesja Popow (Dana Farber Cancer Institute) for providing training data for the UNet model and Zoltan Maliga (Harvard Program in Therapeutic Science) for imaging the human TMA samples and advising on staining protocols. Imaging experiments were supported in part by the Emory University Integrated Cellular Imaging Microscopy Core.
The authors declare no competing interests.
This work was supported by funding from the HSPH Dean’s Fund for Scientific Advancement (K.A.S.), the HSPH National Institute for Environmental Health Sciences (NIEHS) Center (K.A.S., J.S.), R00CA188679 (K.A.S.), R01CA248565 (K.A.S.), R21AI149321 (H.J.), and R01AI148446 (H.J.), Harvard Center for Cancer Systems Pharmacology, U54CA225088 (C.Y.), Precancer Atlas Program, U2CCA233262 (C.Y.), National Cancer Institute, U2CCA233280 (C.Y.), and DARPA Biostasis, W911NF-19-2-0017 (C.Y.).