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Author Notes:

steven.presciutti@emory.edu

Study design: Scott D. Boden, Hicham Drissi, and Steven M. Presciutti. Study conduct: John Rodriguez‐Feo, Lorenzo Fernandes, Anuj Patel, Thanh Doan, and Steven M. Presciutti. Data collection: John Rodriguez‐Feo, Anuj Patel, and Steven M. Presciutti. Data analysis: Lorenzo Fernandes, Thanh Doan, and Steven M. Presciutti.

Data interpretation: Scott D. Boden, Hicham Drissi, and Steven M. Presciutti. Drafting manuscript: Hicham Drissi and Steven M. Presciutti. Approving final version of manuscript: John Rodriguez‐Feo, Lorenzo Fernandes, Anuj Patel, Thanh Doan, Scott D. Boden, Hicham Drissi, and Steven M. Presciutti. Steven M. Presciutti takes responsibility for the integrity of the data analysis.

We acknowledge the assistance of Colleen Oliver for her help performing the animal work, as well as Mesfin Teklemariam and Sreedhara Sangadala for his help performing molecular analyses. We also thank Mila Friedman for her assistance with preparing histology sections.

The authors have no relevant financial conflicts of interest to disclose.

Subjects:

Research Funding:

This work was supported by AOSpine North America as well as Veteran Affairs Career Development Award (IK2‐BX003845).

Keywords:

  • Wnt signaling
  • lumbar spine
  • osteogenesis
  • sclerostin
  • spinal fusion

The temporal and spatial expression of sclerostin and Wnt signaling factors during the maturation of posterolateral lumbar spine fusions

Tools:

Journal Title:

JOR Spine

Volume:

Volume 4, Number 1

Publisher:

, Pages e1100-e1100

Type of Work:

Article | Final Publisher PDF

Abstract:

The bone healing environment in the posterolateral spine following arthrodesis surgery is one of the most challenging in all of orthopedics and our understanding of the molecular signaling pathways mediating osteogenesis during spinal fusion is limited. In this study, the spatial and temporal expression pattern of Wnt signaling factors and inhibitors during spinal fusion was assessed for the first time. Bilateral posterolateral spine arthrodesis with autologous iliac crest bone graft was performed on 21 New Zealand White rabbits. At 1-, 2-, 3-, 4-, and 6-weeks, the expression of sclerostin and a variety of canonical and noncanonical Wnts signaling factors was measured by qRT-PCR from tissue separately collected from the transverse processes, the Outer and Inner Zones of the fusion mass, and the adjancent paraspinal muscle. Immunohistochemistry for sclerostin protein was also performed. Sclerostin and many Wnt factors, especially Wnt3a and Wnt5a, were found to have distinct spatial and temporal expression patterns. For example, harvesting ICBG caused a significant increase in sclerostin expression. Furthermore, the paraspinal muscle immediately adjacent to the transplanted ICBG also had significant increases in sclerostin expression at 3 weeks, suggesting new potential mechanisms for pseudarthroses following spinal arthrodesis. The presented work is the first description of the spatial and temporal expression of sclerostin and Wnt signaling factors in the developing spine fusion, filling an important knowledge gap in the basic biology of spinal fusion and potentially aiding in the development of novel biologics to increase spinal fusion rates.

Copyright information:

© 2020 The Authors. JOR Spine published by Wiley Periodicals LLC. on behalf of Orthopaedic Research Society

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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