Spontaneous local calcium transients regulate oligodendrocyte development in culture through store-operated Ca^2+ entry and release

• Authors: Yanfang Rui, Emory University; Stephanie L. Pollitt, Emory University; Kenneth R. Myers, Emory University; Yue Feng, Emory University; James Zheng, Emory University
• Journal Title: eNeuro
• Volume: Volume 7, Number 4
• Publisher: Society for Neuroscience | 2020-07-01, Pages 1-16
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1523/ENEURO.0347-19.2020
• Subjects: Biology, Neuroscience; Biology, Cell; Biology, Molecular
• Keywords: Ca2+ signaling; internal Ca2+ stores; oligodendrocytes; store-operated Ca2+ entry

Abstract

Oligodendrocytes (OLs) insulate axonal fibers for fast conduction of nerve impulses by wrapping axons of the CNS with compact myelin membranes. Differentiating OLs undergo drastic chances in cell morphology. Bipolar oligodendroglial precursor cells (OPCs) transform into highly ramified multipolar OLs, which then ex-pand myelin membranes that enwrap axons. While significant progress has been made in understanding the molecular and genetic mechanisms underlying CNS myelination and its disruption in diseases, the cellular mechanisms that regulate OL differentiation are not fully understood. Here, we report that developing rat OLs in culture exhibit spontaneous Ca local transients (sCaLTs) in their process arbors in the absence of neurons. Importantly, we find that the frequency of sCaLTs markedly increases as OLs undergo extensive process outgrowth and branching. We further show that sCaLTs are primarily generated through a combination of Ca influx through store-operated Ca entry (SOCE) and Ca release from internal Ca stores. Inhibition of sCaLTs impairs the elaboration and branching of OL processes, as well as substantially reduces the formation of large myelin sheets in culture. Together, our findings identify an important role for spontaneous local Ca signaling in OL development. 2+ 2+ 2+ 2+ 2+ 2+