Detection of viral agents in fecal specimens of monkeys with diarrhea

• Authors: Yuhuan Wang, Emory University; Xinming Tu, Chinese Academy of Medical Sciences and Peking Union Medical College; Charles Humphrey, Emory University; Harold McClure, Emory University; Xi Jiang, University of Cincinnati; Chuan Qin, Chinese Academy of Medical Sciences and Peking Union Medical College; Roger Glass, Emory University; Baoming Jiang, Emory University
• Journal Title: Journal of Medical Primatology
• Volume: Volume 36, Number 2
• Publisher: Wiley | 2007-04-01, Pages 101-107
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1111/j.1600-0684.2006.00167.x
• Subjects: Biology, Zoology; Biology, Virology; Health Sciences, Public Health; Health Sciences, Epidemiology
• Keywords: Science & Technology; Life Sciences & Biomedicine; Veterinary Sciences; Zoology; adenovirus; coronavirus; enterovirus; norovirus; picobirnavirus; rotavirus; Coronavirus-like particles; Non-human primates; Experimental infection; Rotavirus infection; Macaca-nemestrina; Enteric viruses; Rhesus macaques; Norwalk; Feces

Abstract

Background: Diarrheal disease is a major cause of morbidity and mortality in humans and animals, including non human primates. While the diagnostics for gastrointestinal bacterial and parasitic pathogens and their etiological role in disease are well established, little is known about the epidemiology, prevalence and role of viral agents in diarrheal illness among monkeys. Methods: We collected fecal specimens from monkeys with diarrhea that were housed in two primate colonies, the Institute of Laboratory Animal Sciences, Beijing, China and the Yerkes National Primate Research Center, Georgia, USA. We screened these fecal specimens for rotaviruses and enteric adenoviruses 40/41 by using commercial EIA kits (Rotaclone and Adenoclone), enteroviruses by RT-PCR and Southern blot hybridization, and picobirnaviruses by polyacrylamide gel electrophoresis and silver staining. Some of the specimens were examined by EM for coronaviruses and noroviruses. Results: Of the 92 specimens from China, we found 63 (68%) positive for viruses, including enteroviruses (52%), enteric adenoviruses (21%), rotaviruses (20%), and picobirnaviruses (2%). Coronaviruses were detected in some specimens. Mixed infection of two or more viral agents was seen in 23 (25%) specimens. In the US collection, we detected enteroviruses and enteric adenoviruses in 76% (45/59) and 14% (7/50) of the specimens, respectively. Electron microscopy showed norovirus-like particles in some specimens from both colonies. Conclusion: Our findings indicate endemic infections with enteric viruses in monkeys of both colonies. The availability of new simian rotaviruses, enteric adenoviruses, enteroviruses, and coronaviruses and the discovery of noroviruses and picobirnaviruses may allow us to develop better diagnostics for these agents and determine which of these agents are clearly associated with gastroenteritis in monkeys.