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108 Views | 34 Downloads

Author Notes:

prather@emory.edu

Subjects:

Research Funding:

This work is supported by the following awards to P.N.R., VA Merit Award I01 BX001725 and Research Career Scientist Award IK6BX004470, both from the Department of Veterans Affairs, and R01AI072219 from the National Institutes of Health.

Keywords:

  • Acinetobacter baumannii
  • Agrobacterium tumefaciens
  • Biosensor
  • N-Acyl homoserine lactone
  • Quorum sensing
  • Thin-layer chromatography
  • Acinetobacter baumannii
  • Acyl-Butyrolactones
  • Agrobacterium tumefaciens
  • Biosensing Techniques
  • Chemistry Techniques, Analytical
  • Chromatography, Thin Layer
  • Quorum Sensing

Methods for detecting N-Acyl homoserine lactone production in acinetobacter baumannii

Tools:

Journal Title:

Methods Mol Biol

Volume:

Volume 1946

Publisher:

, Pages 253-258

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Acinetobacter baumannii cell-cell signaling is mediated by production and release of N-acyl homoserine lactones (AHLs), primarily N-(3-hydroxydodecanoyl)-l-homoserine lactone (3-hydroxy-C12-HSL). The secretion of this signal can be readily assessed via simple plate-based or thin-layer chromatography-based assays utilizing an Agrobacterium tumefaciens traG-lacZ containing biosensor. In this chapter, we describe methods to assay for secreted N-acyl homoserine lactone production in Acinetobacter baumannii.

Copyright information:

© Springer Science+Business Media, LLC, part of Springer Nature 2019.

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