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To whom correspondence should be addressed. Tel: +1 713 834 6274; Email: xcheng5@mdanderson.org
Correspondence may also be addressed to Taiping Chen. Tel: +1 512 237 9479; Email: tchen2@mdanderscon.org
The authors wish it to be known that, in their opinion, the first two authors should be regarded as Joint First Authors.
Author Contributions: R.R. performed protein purification, DNA-binding experiments, crystallization and X-ray data collection;
J.R.H. performed X-ray data collection and structure determination;
S.H. generated Zbtb24- and Cdca7-deficient mESC lines and stable mESC clones expressing WT and mutant ZBTB24 and performed molecular and cellular experiments to characterize the ICF2 missense mutations;
Y.Z. participated in molecular and cellular experiments;
A.K.S. performed ChIP; L.D.C. performed deep sequencing under the supervision of J.S;
Y.L. and K.L. performed bioinformatics analysis;
H.H. made expression constructs and performed protein purification and DNA binding assays;
C.S.L.K. and R.R. generated R390S mutant;
X.Z. participated in experimental design and discussions throughout the study;
T.C. and X.C. conceived the project, supervised the experimental work and wrote the manuscript.
We thank Z. Ying, B. Liu, H. Zhao, N. Veland, J. Dan and T. Hamidi in the Chen laboratory for discussion and technical assistance, B. Baker at New England Biolabs for synthesizing oligonucleotides, and A. Setili and Y. Cao in the Cheng laboratory for help with protein purification and technique supports.
Conflict of interest statement. None declared.
U.S. National Institutes of Health (NIH) [1R01AI1214030A1 to T.C.; GM049245-24 to X.C.;
CA16672 to the CCSG Cores at MD Anderson Cancer Center];
the Cancer Prevention and Research Institute of Texas [RR160029 to X.C., RP170002 to J.S.]
Thomas Endowment fellowship (to Y.Z.).
Funding for open access charge: NIH.
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.