About this item:

100 Views | 151 Downloads

Author Notes:

Address for correspondence: Fred C. Tenover, Division of Healthcare Quality Promotion, Mailstop G08, Centers for Disease Control and Prevention 1600 Clifton Rd, Atlanta, GA 30333, USA; email: fnt1@cdc.gov

We thank Bette Jensen and Linda McDougal for assistance with PFGE.

Subject:

Research Funding:

Phase 5 of Project ICARE is supported in part by unrestricted grants to the Rollins School of Public Health of Emory University by Astra-Zeneca Pharmaceuticals, bioMérieux, Incorporated, Elan Pharmaceuticals, and Pfizer Incorporated.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Immunology
  • Infectious Diseases
  • HYDROLYZING BETA-LACTAMASE
  • NEW-YORK-CITY
  • PSEUDOMONAS-AERUGINOSA
  • IMIPENEM
  • KPC-2
  • ENTEROBACTERIACEAE
  • EMERGENCE
  • OUTBREAK
  • BROOKLYN
  • SYSTEM

Carbapenem resistance in Klebsielia pneumoniae not detected by automated susceptibility testing

Tools:

Journal Title:

Emerging Infectious Diseases

Volume:

Volume 12, Number 8

Publisher:

, Pages 1209-1213

Type of Work:

Article | Final Publisher PDF

Abstract:

Detecting β-lactamase-mediated carbapenem resistance among Klebsiella pneumoniae isolates and other Enterobacteriaceae is an emerging problem. In this study, 15 blaKPC-positive Klebsiella pneumoniae that showed discrepant results for imipenem and meropenem from 4 New York City hospitals were characterized by isoelectric focusing; broth microdilution (BMD); disk diffusion (DD); and MicroScan, Phoenix, Sensititre, VITEK, and VITEK 2 automated systems. All 15 isolates were either intermediate or resistant to imipenem and meropenem by BMD; 1 was susceptible to imipenem by DD. MicroScan and Phoenix reported 1 (6.7%) and 2 (13.3%) isolates, respectively, as imipenem susceptible. VITEK and VITEK 2 reported 10 (67%) and 5 (33%) isolates, respectively, as imipenem susceptible. By Sensititre, 13 (87%) isolates were susceptible to imipenem, and 12 (80%) were susceptible to meropenem. The VITEK 2 Advanced Expert System changed 2 imipenem MIC results from ≥16 μg/mL to ≤2 μg/mL but kept the interpretation as resistant. The recognition of carbapenem-resistant K. pneumoniae continues to challenge automated susceptibility systems.

Copyright information:

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
Export to EndNote