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Author Notes:

Address correspondence to Sukwan Handali, Division of Parasitic Diseases and Malaria Centers for Disease Control and Prevention Roybal Campus, 1600 Clifton Road, NE, Atlanta, GA 30329. E-mail: ahi0@cdc.gov

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Subjects:

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Public, Environmental & Occupational Health
  • Tropical Medicine
  • SCHISTOSOMA-MANSONI
  • HEPATICA
  • SERODIAGNOSIS
  • ANTIBODIES
  • ANTIGENS
  • ELISA
  • EITB

Development of Two FhSAP2 Recombinant-Based Assays for Immunodiagnosis of Human Chronic Fascioliasis

Tools:

Journal Title:

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

Volume:

Volume 95, Number 4

Publisher:

, Pages 852-855

Type of Work:

Article | Final Publisher PDF

Abstract:

In the United States, infection with Fasciola hepatica has been identified as an emerging disease, primarily in immigrants, refugees, and travelers. The laboratory test of choice for diagnosis of fascioliasis is detection of disease specific antibodies, most commonly uses excretory-secretory antigens for detection of IgG antibodies. Recently, recombinant proteins such as F. hepatica antigen (FhSAP2) have been used to detect IgG antibodies. The glutathione S-transferase (GST)-FhSAP2 recombinant antigen was used to develop Western blot (WB) and fluorescent bead-based (Luminex) assays to detect F. hepatica total IgG and IgG4 antibodies. The sensitivity and specificity of GST-FhSAP2 total IgG and IgG4 WB were similar at 94% and 98%, respectively. For the IgG Luminex assay, the sensitivity and specificity were 94% and 97%, and for the IgG4, the values were 100% and 99%, respectively. In conclusion, the GST-FhSAP2 antigen performs well in several assay formats and can be used for clinical diagnosis.

Copyright information:

© 2016 by The American Society of Tropical Medicine and Hygiene.

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