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Author Notes:

Brian Oliver, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Room 3339, 50 South Dr., Bethesda, Maryland 20814. E-mail: briano@nih.gov

Dorothy Lerit, Department of Cell Biology, Emory University School of Medicine, Room 444 Whitehead Bldg., 615 Michael St., Atlanta, Georgia 30322. E-mail: dlerit@emory.edu

L.B., E.A.C., K.R.C., B.O., and D.A.L. designed the project and analyzed data.

L.B., K.R.C., C.W., and K.J.T.V. performed genetics. L.B. and H.Y. performed genomics. J.F. performed western blotting. L.B., E.A.C., C.W., and D.A.L. performed imaging. L.B., B.O., and D.A.L. wrote the manuscript. All authors reviewed data and provided feedback on the manuscript.

See publication for list of acknowledgments.

Subjects:

Research Funding:

This research was supported in part by the Intramural Research Program of the NIH NIDDK (awarded to B.O.).

D.A.L. and E.A.C. were supported by NIH grant 5K22 HL-126922 (D.A.L.).

L.B. was supported by the NIH Graduate Partners Program.

K.J.T.V. was supported by Baylor College of Medicine, the Albert and Margaret Alkek Foundation, the McNair Medical Institute at The Robert and Janice McNair Foundation, the March of Dimes Foundation (#1-FY14-315), the Foundation for Angelman Syndrome Therapeutics (FT2016-002), the Cancer Prevention and Research Institute of Texas (R1313), and NIH grants 1R21 HG-006726, 1R21 GM-110190, 1R21 OD-022981, and R01 GM-109938).

C.W. and K.R.C. were supported by the Office of the NIH Director, the National Institute of General Medical Sciences, and the NICHD (P40 OD-018537).

K.R.C. was supported by the National Center for Resource (R24RR014106) and the National Science Foundation (DBI-9816125).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Genetics & Heredity
  • sov zinc-finger
  • heterochromatin
  • gene expression
  • HP1a
  • oogenesis
  • position-effect variegation
  • POSITION-EFFECT VARIEGATION
  • NONHISTONE CHROMOSOMAL-PROTEIN
  • GENE-EXPRESSION
  • BINDING PROTEIN
  • RNA-SEQ
  • MELANOGASTER
  • METHYLATION
  • PIRNA
  • SUPPRESSOR
  • OOGENESIS

Drosophila Heterochromatin Stabilization Requires the Zinc-Finger Protein Small Ovary

Tools:

Journal Title:

GENETICS

Volume:

Volume 213, Number 3

Publisher:

, Pages 877-895

Type of Work:

Article | Final Publisher PDF

Abstract:

Heterochromatin-mediated repression is essential for controlling the expression of transposons and for coordinated cell type-specific gene regulation. The small ovary (sov) locus was identified in a screen for female-sterile mutations in Drosophila melanogaster, and mutants show dramatic ovarian morphogenesis defects. We show that the null sov phenotype is lethal and map the locus to the uncharacterized gene CG14438, which encodes a nuclear zinc-finger protein that colocalizes with the essential Heterochromatin Protein 1 (HP1a). We demonstrate Sov functions to repress inappropriate gene expression in the ovary, silence transposons, and suppress position-effect variegation in the eye, suggesting a central role in heterochromatin stabilization.

Copyright information:

© 2019 Genetics Society of America. All rights reserved.

This is an Open Access work distributed under the terms of the Creative Commons Universal : Public Domain Dedication License (https://creativecommons.org/publicdomain/zero/1.0/).
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