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Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Critical Care Medicine
  • Hematology
  • Surgery
  • Peripheral Vascular Disease
  • General & Internal Medicine
  • Cardiovascular System & Cardiology
  • Animal welfare
  • best practices
  • preclinical models
  • sepsis
  • study design
  • INTERNATIONAL CONSENSUS DEFINITIONS
  • FACTOR PATHWAY INHIBITOR
  • CHRONIC CRITICAL ILLNESS
  • CECAL LIGATION
  • SEPTIC SHOCK
  • RECEPTOR ANTAGONIST
  • ANIMAL RESEARCH
  • DOUBLE-BLIND
  • PHASE-III
  • MORTALITY

PART I: MINIMUM QUALITY THRESHOLD IN PRECLINICAL SEPSIS STUDIES (MQTiPSS) FOR STUDY DESIGN AND HUMANE MODELING ENDPOINTS

Tools:

Journal Title:

SHOCK

Volume:

Volume 51, Number 1

Publisher:

, Pages 10-22

Type of Work:

Article

Abstract:

© 2018 by the Shock Society. Preclinical animal studies are mandatory before new treatments can be tested in clinical trials. However, their use in developing new therapies for sepsis has been controversial because of limitations of the models and inconsistencies with the clinical conditions. In consideration of the revised definition for clinical sepsis and septic shock (Sepsis-3), a Wiggers-Bernard Conference was held in Vienna in May 2017 to propose standardized guidelines on preclinical sepsis modeling. The participants conducted a literature review of 260 most highly cited scientific articles on sepsis models published between 2003 and 2012. The review showed, for example, that mice were used in 79% and euthanasia criteria were defined in 9% of the studies. Part I of this report details the recommendations for study design and humane modeling endpoints that should be addressed in sepsis models. The first recommendation is that survival follow-up should reflect the clinical time course of the infectious agent used in the sepsis model. Furthermore, it is recommended that therapeutic interventions should be initiated after the septic insult replicating clinical care. To define an unbiased and reproducible association between a new treatment and outcome, a randomization and blinding of treatments as well as inclusion of all methodological details in scientific publications is essential. In all preclinical sepsis studies, the high standards of animal welfare must be implemented. Therefore, development and validation of specific criteria for monitoring pain and distress, and euthanasia of septic animals, as well as the use of analgesics are recommended. A set of four considerations is also proposed to enhance translation potential of sepsis models. Relevant biological variables and comorbidities should be included in the study design and sepsis modeling should be extended to mammalian species other than rodents. In addition, the need for source control (in case of a defined infection focus) should be considered. These recommendations and considerations are proposed as "best practices" for animal models of sepsis that should be implemented.
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