Regulation of Expression and Function of Scavenger Receptor Class B, Type I (SR-BI) by Na+/H+ Exchanger Regulatory Factors (NHERFs)

• Authors: Zhigang Hu, Veterans Affairs Palo Alto Health Care System; Jie Hu, Veterans Affairs Palo Alto Health Care System; Zhonghua Zhang, Veterans Affairs Palo Alto Health Care System; Wen-Jun Shen, Veterans Affairs Palo Alto Health Care System; Changhyon Yun, Emory University; Catherine H Berlot, Weis Center for Research; Fredric B Kraemer, Veterans Affairs Palo Alto Health Care System; Salman Azhar, Veterans Affairs Palo Alto Health Care System
• Journal Title: Journal of Biological Chemistry
• Volume: Volume 288, Number 16
• Publisher: American Society forBiochemistry and Molecular Biology | 2013-04-19, Pages 11416-11435
• Type of Work: Article | Final Publisher PDF
• Publisher DOI: http://doi.org/10.1074/jbc.M112.437368
• Subjects: Health Sciences, Medicine and Surgery; Health Sciences, General
• Keywords: Science & Technology; Life Sciences & Biomedicine; Biochemistry & Molecular Biology; HIGH-DENSITY-LIPOPROTEIN; CHOLESTERYL ESTER UPTAKE; PROTEIN-COUPLED-RECEPTORS; DOMAIN-CONTAINING PROTEIN; CAMP-MEDIATED INHIBITION; OVARIAN GRANULOSA-CELLS; SELECTIVE UPTAKE; H+ EXCHANGER; PDZ PROTEINS; BETA(2)-ADRENERGIC RECEPTOR

Abstract

Background: SR-BI mediates selective delivery of lipoprotein-CE to the liver, adrenals, and gonads for product formation. Results: NHERFs interact with and down-regulate SR-BI protein levels to inhibit selective CE uptake and steroidogenesis. Conclusion: Two protein domains (PDZ and MERM) are required for NHERF1/2-mediated inhibition of SR-BI expression and function. Significance: This work reveals a novel mechanism of translational/posttranslational regulation of SR-BI. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.