194 Views | 236 Downloads
Vincent P. Conticello: vcontic@emory.edu
S.A.H. and V.P.C. designed research; S.A.H., F.W., S.W., M.A.B.K., A.O., J.S.W., X.Z., and E.H.E. performed research; S.A.H., F.W., S.W., M.A.B.K., T.O., J.S.W., X.Z., E.H.E., and V.P.C. analyzed data; and S.A.H., F.W., S.W., M.A.B.K., T.O., A.O., J.S.W., X.Z., E.H.E., and V.P.C. wrote the paper.
The authors declare no conflict of interest.
This research was, in part, supported by the National Cancer Institute’s National Cryo-EM Facility at the Frederick National Laboratory for Cancer Research under contract HSSN261200800001E.
This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under contract DE-AC02-06CH11357.
This research used the National Cancer Institute’s National Cryo-EM Facility at the Frederick National Laboratory for Cancer Research.
The cryo-EM work on the HEAT_R1 filaments was conducted at the Molecular Electron Microscopy Core facility at the University of Virginia, which is supported by the School of Medicine and built with NIH grant G20-RR31199
The Titan Krios within that core was funded by NIH shared instrumentation (SIG) grant S10-RR025067; and the Falcon II direct detector was funded by NIH SIG S10-OD018149.
This work was supported by NSF Division of Material Research grant 1533958 (to V.P.C. and E.H.E.).
© 2019 National Academy of Sciences. All rights reserved.