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Author Notes:

Sarah Ingersoll, PhD, Center for Diagnostics & Therapeutics, Department of Biology, Georgia State University, PO Box 5090, Atlanta, GA 30302-5090 phone: (404)-413-3598 fax: (404)-413-3580; singersoll@gsu.edu

We dedicate this article to the memory of Dr. Shanthi V. Sitaraman, a brilliant scientist, dedicated physician, passionate humanitarian and dearest friend.

Authors reported no conflicts of interest.

Subject:

Research Funding:

This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases Grants, DK06411.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Gastroenterology & Hepatology
  • A(2B) receptor
  • Inflammation
  • Nanoparticle therapy
  • INFLAMMATORY-BOWEL-DISEASE
  • ADENOSINE RECEPTORS
  • T-CELLS
  • INTESTINAL INFLAMMATION
  • PURINERGIC RECEPTORS
  • CHLORIDE SECRETION
  • CUTTING EDGE
  • MICE
  • RESPONSES
  • PROTECTS

A(2B)AR expression in non-immune cells plays an important role in the development of murine colitis

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Journal Title:

Digestive and Liver Disease

Volume:

Volume 44, Number 10

Publisher:

, Pages 819-826

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Background: Adenosine, an endogenous purine nucleoside, is involved in several physiological functions. We have previously shown that A 2BAR plays a pro-inflammatory role during colitis. Aims: Our goals were to determine if A 2BAR expression was necessary on immune cells/non-immune cells during colitis and if A 2BAR was a suitable target for treating intestinal inflammation. Methods: Wild-type and A 2BAR knockout mice were utilized in bone marrow transplants to explore the importance of immune/non-immune A 2BAR expression during the development of colitis. Additionally, a T-cell transfer model of colitis was used in Rag1 knockout or A 2BAR/RAG1 double knockout recipients. Finally, A 2BAR small interfering RNA nanoparticles were administered to dextran sodium sulphate-treated mice. Results: Wild-type mice receiving wild-type or knockout bone marrow developed severe colitis after dextran sodium sulphate treatment, whereas colitis was significantly attenuated in knockout mice receiving wild-type or knockout bone marrow. Colitis induced in Rag1 knockout animals was attenuated in A 2BAR/RAG1 double knockout recipients. Animals receiving nanoparticles exhibited attenuated parameters of colitis severity compared to mice receiving control nanoparticles. Conclusions: Our results suggest that A 2BAR on non-immune cells plays an important role for the induction of colitis and targeting A 2BAR expression during colitis may be useful for alleviating symptoms of intestinal inflammation.

Copyright information:

© 2012 Editrice Gastroenterologica Italiana S.r.l.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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