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Author Notes:

Xinsheng Zhang: AIDS Vaccine Design and Development Laboratory, International AIDS Vaccine Initiative (IAVI), Brooklyn Army Terminal, 140 58th Street, Building A, 8th Floor, Unit 8J, Brooklyn, NY 11220, USA. Tel.: + 1 646 381 8037; fax: + 1 718 765 1870. xzhang@iavi.org

The authors are grateful for Emmanuel Cormier, Laura Sharpe and Brendan McAtarsney for serum sample management.

We also thank Wayne Koff, Rick King, Josephine Cox and Beth Rasmussen for helpful suggestions and advice; and acknowledge Heather Arendt and Joanne DeStefano for critical reading of the manuscript.

The authors also thank Shinji Ohno and Makoto Takeda from Kyushu University for providing the 2F4 mouse monoclonal antibody.

Subjects:

Research Funding:

IAVI's work is made possible by generous support from many donors including: the Bill & Melinda Gates Foundation; the Ministry of Foreign Affairs of Denmark; Irish Aid; the Ministry of Finance of Japan; the Ministry of Foreign Affairs of the Netherlands; the Norwegian Agency for Development Cooperation (NORAD); the United Kingdom Department for International Development (DFID), and the United States Agency for International Development (USAID).

This study is made possible by the generous support of the American people through USAID and is supported by the Bill and Melinda Gates Foundation Collaboration for AIDS Vaccine Discovery (CAVD).

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Virology
  • Canine distemper virus
  • Measles virus
  • Plaque reduction neutralization test
  • Neutralizing antibody
  • Hemagglutinin
  • Pre-existing immunity
  • Cross-neutralization
  • MEASLES-VIRUS
  • IMMUNOLOGICAL RELATIONSHIPS
  • IMMUNE-RESPONSE
  • LIVE MEASLES
  • VACCINE
  • ANTIBODIES
  • OUTBREAK
  • INFECTIONS
  • MONKEYS
  • FUSION

Canine distemper virus neutralization activity is low in human serum and it is sensitive to an amino acid substitution in the hemagglutinin protein

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Journal Title:

Virology

Volume:

Volume 482

Publisher:

, Pages 218-224

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Serum was analyzed from 146 healthy adult volunteers in eastern Africa to evaluate measles virus (MV) and canine distemper virus (CDV) neutralizing antibody (nAb) prevalence and potency. MV plaque reduction neutralization test (PRNT) results indicated that all sera were positive for MV nAbs. Furthermore, the 50% neutralizing dose (ND50) for the majority of sera corresponded to antibody titers induced by MV vaccination. CDV nAbs titers were low and generally were detected in sera with high MV nAb titers. A mutant CDV was generated that was less sensitive to neutralization by human serum. The mutant virus genome had 10 nucleotide substitutions, which coded for single amino acid substitutions in the fusion (F) and hemagglutinin (H) glycoproteins and two substitutions in the large polymerase (L) protein. The H substitution occurred in a conserved region involved in receptor interactions among morbilliviruses, implying that this region is a target for cross-reactive neutralizing antibodies.

Copyright information:

© 2015 Elsevier Inc. All rights reserved.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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