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Author Notes:

E-mail address: hfa5@cdc.gov

Conceived and designed the experiments: NGR DC.

Performed the experiments: NGR SW SS MM JB CC.

Analyzed the data: NGR DC CD MM YN.

Contributed reagents/materials/analysis tools: GE WD JS SZ KK.

Wrote the paper: NGR DC CD CH VO.

Manuscript edits: DC ID.

The authors have declared that no competing interests exist.


Research Funding:

The authors have no support or funding to report.


  • Science & Technology
  • Multidisciplinary Sciences
  • Science & Technology - Other Topics

The Pox in the North American Backyard: Volepox Virus Pathogenesis in California Mice (Peromyscus californicus)

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Journal Title:



Volume 7, Number 8


, Pages e43881-e43881

Type of Work:

Article | Final Publisher PDF


Volepox virus (VPXV) was first isolated in 1985 from a hind foot scab of an otherwise healthy California vole (Microtus californicus). Subsequent surveys in San Mateo County, CA, revealed serological evidence suggesting that VPXV is endemic to this area, and a second viral isolate from a Pinyon mouse (Peromyscus truei) was collected in 1988. Since then, few studies have been conducted regarding the ecology, pathology, and pathogenicity of VPXV, and its prevalence and role as a potential zoonotic agent remain unknown. To increase our understanding of VPXV disease progression, we challenged 24 California mice (Peromyscus californicus) intranasally with 1.6×103 PFU of purified VPXV. By day five post infection (pi) we observed decreased activity level, conjunctivitis, ruffled hair, skin lesions, facial edema, and crusty noses. A mortality rate of 54% was noted by day eight pi. In addition, internal organ necrosis and hemorrhages were observed during necropsy of deceased or euthanized animals. Viral loads in tissues (brain, gonad, kidney, liver, lung, spleen, submandibular lymph node, and adrenal gland), bodily secretions (saliva, and tears), and excretions (urine, and/or feces) were evaluated and compared using real time-PCR and tissue culture. Viral loads measured as high as 2×109 PFU/mL in some organs. Our results suggest that VPXV can cause extreme morbidity and mortality within rodent populations sympatric with the known VPXV reservoirs.

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© 2012.

This is an Open Access work distributed under the terms of the Creative Commons Universal : Public Domain Dedication License (http://creativecommons.org/publicdomain/zero/1.0/).

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