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Author Notes:
Correspondence to: Shoichiro Ono, Department of Pathology, Emory University, 615 Michael Street, Whitehead Research Building, Room 105N, Atlanta, GA 30322, USA, Tel: (404) 727-3916, Fax: (404) 727-8538, sono@emory.edu
Subject:
Research Funding:
The C. elegans strain was provided by the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources.
This work was supported by NIH grant AR48615 to S. O. and by the American Heart Association to D. P.
Keywords:
- Actin
- C. elegans
- nematodes
- purification
- polymerization
Biochemical and cell biological analysis of actin in the nematode Caenorhabditis elegans
Abstract:
The nematode Caenorhabditis elegans has long been a useful model organism for muscle research. Its body wall muscle is obliquely striated muscle and exhibits structural similarities with vertebrate striated muscle. Actin is the core component of the muscle thin filaments, which are highly ordered in sarcomeric structures in striated muscle. Genetic studies have identified genes that regulate proper organization and function of actin filaments in C. elegans muscle, and sequence of the worm genome has revealed a number of conserved candidate genes that may regulate actin. To precisely understand the functions of actin-binding proteins, such genetic and genomic studies need to be complemented by biochemical characterization of these actin-binding proteins in vitro. This article describes methods for purification and biochemical characterization of actin from C. elegans. Although rabbit muscle actin is commonly used to characterize actin-binding proteins from many eukaryotic organisms, we detect several quantitative differences between C. elegans actin and rabbit muscle actin, highlighting that use of actin from an appropriate source is important in some cases. Additionally, we describe probes for cell biological analysis of actin in C. elegans.
Copyright information:
© 2011 Elsevier Inc. All rights reserved.
This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommerical-NoDerivs 3.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/3.0/).
