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Author Notes:

Correspondence: Shawn Hochman, Department of Physiology, Emory University School of Medicine, 615 Michael Street, Atlanta, GA, 30322, USA; Email: shochm2@emory.edu.

Acknowledgments: We thank Dr Dapeng Cui for assistance with real-time PCR.

Disclosures: All experimental procedures complied with the “Principles of animal care,” publication No. 86-23, revised 1985 by the NIH, and the Emory Institutional Animal Care and Use Committee.

Subjects:

Research Funding:

Funding for this study was awarded from the National Institutes of Health, NINDS, Grant NS045248.

Keywords:

  • Monoamines
  • Real-time PCR
  • In situ hybridization
  • Restless Legs Syndrome
  • Dopamine

Unaltered D1, D2, D4, and D5 dopamine receptor mRNA expression and distribution in the spinal cord of the D3 receptor knockout mouse

Tools:

Journal Title:

Journal of Comparative Physiology A

Volume:

Volume 194, Number 11

Publisher:

, Pages 957-962

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Dopamine (DA) acts through five receptor subtypes (D1–D5). We compared expression levels and distribution patterns of all DA mRNA receptors in the spinal cord of wild-type (WT) and loss of function D3 receptor knockout (D3KO) animals. D3 mRNA expression was increased in D3KO, but no D3 receptor protein was associated with cell membranes, supporting the previously reported lack of function. In contrast, mRNA expression levels and distribution patterns of D1, D2, D4, and D5 receptors were similar between WT and D3KO animals. We conclude that D3KO spinal neurons do not compensate for the loss of function of the D3 receptor with changes in the other DA receptor subtypes. This supports use of D3KO animals as a model to provide insight into D3 receptor dysfunction in the spinal cord.

Copyright information:

© Springer-Verlag 2008

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