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Author Notes:

Address correspondence and reprint requests to Dr. Rafi Ahmed, 1510 Clifton Road., G211, Atlanta, GA 30322. rahmed@.emory.edu

Acknowledgments We thank K. Araki and R. Aubert for input and critical reading of the manuscript and A. Popkowski for technical assistance.

We also thank Mario Roederer, National Institutes of Health, Vaccine Research Center, for providing the PESTLE and SPICE software.

Disclosures The authors have no financial conflict of interest.

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Research Funding:

This work was supported by National Institutes of Health (NIH) U19 Grant AI057266 (to R.A.) and in part by Sanofi-Pasteur, Lyon, France. C.M.R. receives support from the Greenberg Medical Research Institute, the Starr Foundation, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative (grant identification nos. 334 and 574), General Clinical Research Center Grant M01-RR00102 (to Rockefeller University Hospital), and Center for Translational Science Award Grant 1UL1 RR024143-01 (to Rockefeller University Hospital) from the NIH National Center for Research Resources.

The Yellow Fever Virus Vaccine Induces a Broad and Polyfunctional Human Memory CD8+ T Cell Response1

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Journal Title:

Journal of Immunology

Volume:

Volume 183, Number 12

Publisher:

, Pages 7919-7930

Type of Work:

Article | Post-print: After Peer Review

Abstract:

The live yellow fever vaccine (YF-17D) offers a unique opportunity to study memory CD8+ T cell differentiation in humans following an acute viral infection. We have performed a comprehensive analysis of the virus-specific CD8+ T cell response using overlapping peptides spanning the entire viral genome. Our results showed that the YF-17D vaccine induces a broad CD8+ T cell response targeting several epitopes within each viral protein. We identified a dominant HLA-A2-restricted epitope in the NS4B protein and used tetramers specific for this epitope to track the CD8+ T cell response over a 2 year period. This longitudinal analysis showed the following. 1) Memory CD8+ T cells appear to pass through an effector phase and then gradually down-regulate expression of activation markers and effector molecules. 2) This effector phase was characterized by down-regulation of CD127, Bcl-2, CCR7, and CD45RA and was followed by a substantial contraction resulting in a pool of memory T cells that re-expressed CD127, Bcl-2, and CD45RA. 3) These memory cells were polyfunctional in terms of degranulation and production of the cytokines IFN-γ, TNF-α, IL-2, and MIP-1β. 4) The YF-17D-specific memory CD8+ T cells had a phenotype (CCR7−CD45RA+) that is typically associated with terminally differentiated cells with limited proliferative capacity (TEMRA). However, these cells exhibited robust proliferative potential showing that expression of CD45RA may not always associate with terminal differentiation and, in fact, may be an indicator of highly functional memory CD8+ T cells generated after acute viral infections.

Copyright information:

© 2009 by The American Association of Immunologists, Inc.

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