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Author Notes:

John R. Hepler, Ph.D., Rollins Research Center, 1510 Clifton Road, Suite G205, Atlanta, GA 30322-3090, Phone: (404) 727-3641, Fax: (404) 727-0365, jhepler@emory.edu.

P.R.E., S.E.L., and J.R.H. designed research; P.R.E. and S.E.L. performed experiments; P.R.E. analyzed and interpreted data; P.R.E. wrote the manuscript; P.R.E., Y.S., and J.R.H critically revised the manuscript.

We thank Marla Gearing and Deborah Cooper for exceptional technical assistance and helpful discussion of data.

We also thank Drs. Belvin Gong and Bianca Bautista of the NIH/NINDS-sponsored NeuroMabs facility at the University of California-Davis for their outstanding work in developing the anti-RGS14 monoclonal antibody used in these studies.

The authors declare no conflict of interest.

Subjects:

Research Funding:

These studies were supported by grants NIH/NINDS 5R01 NS37112 and NIH/NINDS 1R21NS074975 both awarded to JRH.

The work was supported by NIH T32GM008605 training grant awarded to Emory Graduate Program in Neuroscience.

This work was also supported by the National Center for Research Resources P51RR000165 and the Office of Research Infrastructure Programs / OD P51OD011132 to the Yerkes National Primate Center.

This research project was supported in part by the histopathology core of the Emory Neuroscience NINDS Core Facilities grant, P30NS055077.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Neurosciences
  • Zoology
  • Neurosciences & Neurology
  • RGS14
  • hippocampus
  • hippocampal CA2
  • synaptic plasticity
  • RGS proteins
  • SYNAPTIC PLASTICITY
  • CA2 NEURONS
  • RAT-BRAIN
  • HIPPOCAMPAL
  • G-ALPHA(I)
  • LOCALIZATION
  • ISCHEMIA
  • DOMAIN
  • MEMORY
  • ALPHA

Postnatal Developmental Expression of Regulator of G Protein Signaling 14 (RGS14) in the Mouse Brain

Tools:

Journal Title:

Journal of Comparative Neurology

Volume:

Volume 522, Number 1

Publisher:

, Pages 186-203

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Regulator of G protein signaling 14 (RGS14) is a multifunctional scaffolding protein that integrates G protein and mitogen-activated protein kinase (MAPK) signaling pathways. In the adult mouse brain, RGS14 mRNA and protein are found almost exclusively in hippocampal CA2 neurons. We have shown that RGS14 is a natural suppressor of CA2 synaptic plasticity and hippocampal-dependent learning and memory. However, the protein distribution and spatiotemporal expression patterns of RGS14 in mouse brain during postnatal development are unknown. Here, using a newly characterized monoclonal anti-RGS14 antibody, we demonstrate that RGS14 protein immunoreactivity is undetectable at birth (P0), with very low mRNA expression in the brain. However, RGS14 protein and mRNA are upregulated during early postnatal development, with protein first detected at P7, and both increasing over time until reaching highest sustained levels throughout adulthood. Our immunoperoxidase data demonstrate that RGS14 protein is expressed in regions outside of hippocampal CA2 during development including the primary olfactory areas, the anterior olfactory nucleus and piriform cortex, and the olfactory associated orbital and entorhinal cortices. RGS14 is also transiently expressed in neocortical layers II/III and V during postnatal development. Finally, we show that RGS14 protein is first detected in the hippocampus at P7, with strongest immunoreactivity in CA2 and fasciola cinerea and sporadic immunoreactivity in CA1; labeling intensity in hippocampus increases until adulthood. These results show that RGS14 mRNA and protein are upregulated throughout postnatal mouse development, and RGS14 protein exhibits a dynamic localization pattern that is enriched in hippocampus and primary olfactory cortex in the adult mouse brain.

Copyright information:

© 2013 Wiley Periodicals, Inc.

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