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Author Notes:

Correspondence: Kerry Ressler, MD, PhD, Psychiatry and Behavioral Sciences, Yerkes Research Center, 954 Gatewood Dr, NE, Atlanta, GA 30319; Email: kressle@emory.edu

Authors' Contributions: DAG and OPK contributed equally to this work

Subjects:

Research Funding:

Funding for this project was provided by two Venture Grants from the Science and Technology Center (The Center for Behavioral Neuroscience of the National Science Foundation under Agreement No. IBN-9876754).

Support was also provided by the National Institutes of Health (MH071537, DA019624), the Burroughs Wellcome Fund and the National Primate Research Center base grant #RR-00165.

Keywords:

  • Diffusion Tensor Imaging (DTI)
  • Infralimbic/Prelimbic
  • Mouse
  • Prefrontal Cortex
  • Amygdala

A DTI Tractography analysis of Infralimbic and Prelimbic Connectivity in the Mouse using High-throughput MRI

Tools:

Journal Title:

NeuroImage

Volume:

Volume 63, Number 2

Publisher:

, Pages 800-811

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Background High throughput, brain-wide analysis of neural circuit connectivity is needed to understand brain function across species. Combining such tractography techniques with small animal models will allow more rapid integration of systems neuroscience with molecular genetic, behavioral, and cellular approaches. Methods We collected DTI and T2 scans on 3 series of 6 fixed mouse brains ex vivo in a 9.4 Tesla magnet. The DTI analysis of ten mouse brains focused on comparing prelimbic (PL) and Infralimbic (IL) probabilistic tractography. To validate the DTI results a preliminary set of 24 additional mice were injected with BDA into the IL and PL. The DTI results and preliminary BDA results were also compared to previously published rat connectivity. Results We focused our analyses on the connectivity of the mouse prelimbic (PL) vs. infralimbic (IL) cortices. We demonstrated that this DTI analysis is consistent across scanned mice, with prior analyses of rat IL/PL connectivity, and with mouse PL and IL projections using the BDA tracer. Conclusions High-throughput ex vivo DTI imaging in the mouse delineated both common and differential connectivity of the IL and PL cortex. The scanning methodology provided a balance of tissue contrast, signal-to-noise ratio, resolution and throughput. Our results are largely consistent with previously published anterograde staining techniques in rats, and the preliminary tracer study of the mouse IL and PL provided here.

Copyright information:

© 2012 Elsevier Inc. All rights reserved.

This is an Open Access work distributed under the terms of the Creative Commons Attribution-NonCommerical-NoDerivs 3.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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