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Author Notes:

Ichiro Matsumura: imatsum@emory.edu

We thank the Bacillus Genetic Stock Center for GsNUB3621, the pNW33N plasmid, and their protocols.

We also thank the Emory Integrated Genomics Core for their service and Paul Doetsch for the use of his spectrofluorimeter.

Subject:

Research Funding:

This work was supported by the National Institute of General Medicine at the National Institutes for Health. KB and IM were supported by R01 GM086824 and KB was also supported by 5T32GM008490-19.

Keywords:

  • Science & Technology
  • Life Sciences & Biomedicine
  • Biotechnology & Applied Microbiology
  • Geobacillus stearothermophilus
  • Facultative thermophile
  • Genome
  • Expression system
  • BACILLUS-STEAROTHERMOPHILUS
  • ALPHA-GALACTOSIDASE
  • GENE-EXPRESSION
  • GENOME SEQUENCE
  • SHUTTLE VECTOR
  • CLONING
  • IDENTIFICATION
  • PURIFICATION
  • STABILITY
  • EVOLUTION

Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering

Tools:

Journal Title:

Applied Microbiology and Biotechnology

Volume:

Volume 98, Number 15

Publisher:

, Pages 6715-6723

Type of Work:

Article | Post-print: After Peer Review

Abstract:

Metabolic engineers develop inexpensive enantioselective syntheses of high-value compounds, but their designs are sometimes confounded by the misfolding of heterologously expressed proteins. Geobacillus stearothermophilus NUB3621 is a readily transformable facultative thermophile. It could be used to express and properly fold proteins derived from its many mesophilic or thermophilic Bacillaceae relatives or to direct the evolution of thermophilic variants of mesophilic proteins. Moreover, its capacity for high-temperature growth should accelerate chemical transformation rates in accordance with the Arrhenius equation and reduce the risks of microbial contamination. Its tendency to sporulate in response to nutrient depletion lowers the costs of storage and transportation. Here, we present a draft genome sequence of G. stearothermophilus NUB3621 and describe inducible and constitutive expression plasmids that function in this organism. These tools will help us and others to exploit the natural advantages of this system for metabolic engineering applications.

Copyright information:

© 2014 Springer-Verlag.

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