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Author Notes:

Correspondence should be addressed to Chi-Bin Chien, Department of Neurobiology and Anatomy, 401 MREB, University of Utah Medical Center, 20 North 1900 East, Salt Lake City, UT 84132-3401. chi-bin.chien@neuro.utah.edu

We thank H. Okamoto and H. Baier for providing the isl1:gfp and brn3c:gap43-gfp fish lines; M. Bak-Maier and S. Fraser for discussing unpublished work, reading the manuscript, and pointing out the misspliced RNA generated by DCC-MO3; Lara Hutson for designing DCC-MO1; and K. Kwan, A. Pittman, K. Rasband, and J. Rosenthal for help with quantitation.

Thanks to A. Alvarado-Sanchez, R. Dorsky, D. Grunwald, T. Piotrowski, and all members of the Chien laboratory for discussions and support and to R. Dorsky and J. Bonkowsky for comments on this manuscript.


Research Funding:

This work was supported by National Institutes of Health Grant R01 EY12873 (C.-B.C.) and March of Dimes Basil O'Connor Award 5-FY02-270 (I.S.).


  • Animals
  • Animals, Genetically Modified
  • DCC Receptor
  • Dendrites
  • Fetal Tissue Transplantation
  • Nerve Growth Factors
  • Netrin-1
  • Neurons, Efferent
  • Receptors, Cell Surface
  • Rhombencephalon
  • Signal Transduction
  • Tumor Suppressor Proteins
  • Zebrafish
  • Zebrafish Proteins

Netrin/DCC signaling controls contralateral dendrites of octavolateralis efferent neurons


Journal Title:

Journal of Neuroscience Nursing


Volume 26, Number 51


, Pages 13328-13337

Type of Work:

Article | Final Publisher PDF


The guidance molecule Netrin and its receptor DCC (deleted in colorectal cancer) attract commissural axons toward the midline en route to their final destination. To test whether these molecules can also guide dendrites, we studied the contralateral dendrites of zebrafish octavolateralis efferent (OLe) neurons, which are unusual in that they navigate toward and cross the midline. We found that, at the time of dendrite outgrowth, OLe neurons express dcc, and the hindbrain midline expresses netrin1. Knocking down dcc or netrin1 function by injecting antisense morpholino oligonucleotides prevented OLe contralateral dendrites from crossing the midline, showing that dcc and netrin1 are necessary for dendrite guidance or formation. Furthermore, by transplanting cells from dcc morphants into wild-type embryos and vice versa, we demonstrated that dcc acts cell autonomously in OLe dendrites. This work is the first evidence that Netrin/DCC signaling acts in dendrites in a vertebrate system.

Copyright information:

Copyright © 2006 Society for Neuroscience.

This is an Open Access work distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
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